Research On Integrated PCR Excitation Detection System

Polymerase chain reaction was invented by the United States Mullis in 1985. It is a molecular biology technique for the in vitro amplification of specific DNA fragments. Based on the polymerase chain theory, PCR instrument was invented. PCR instrument developed through qualitative to quantitative process. Current PCR technology can already compute DNA quantitatively in the reactants. Fluorescence detection system is one of the core components to implement the process. It detects the PCR reaction by the changes of the fluorescence intensity.Firstly, in this paper, based on the fluorescent light principle and characteristics, we use fluorescence analysis and the characteristics of excitation and emission spectra in quantitative PCR fluorescent reagents. On the basis of oblique fluorescence detection method, we preliminary design a multi-band real-time detection system with four channel. In this system, we use a through-hole LED as the excitation light source and the PIN photodiode as the detector. Besides this, we design four sets of filters to separate the band.Then according to the principle of non-imaging optics, we use simulation software of Tracepro to simulate and optimize the excitation and detection system, so that to achieve an ideal effect of the solar energy utilization. During this simulation, we build a model and set the light source and the material properties to achieve optimization.Finally, experimental results prove the feasibility of this fluorescence detection system. In this experiment, we can detect fluorescence signal emitted from FAM, and find a linear relationship between the fluorescence intensity and the concentration of the reagents by different fluorescence intensity from different concentration ratio of FAM.