The Arabidopsis Thaliana Transformation By Populus Euphratica Genome Fragment And Phenotypic Analysis

Populus euphratica is an oldest branch of the Populus, which has a strong ability to adapt to the environment, and is an excellent material for research of woody plant tolerance to salinity, drought, high temperature and other extreme abiotic traits. With the rapid development of genetic engineering technology, the major area of Populus euphratica research from the tissue culture as the center of a series of physiological and biochemical experiment extends to the field of molecular. Studies have shown that plant genetic template gene clone of Populus resistance to saline model is feasible in, but because of the complexity of species differences in the regulation and control of gene expression, not deep and thorough to elucidate the Populus euphratica cope with stress environment of the molecular mechanism. With the update of technology and new achievements in scientific research, mining of Populus euphratica gene analysis from a single gene, single signal pathway development to multiple genes and signaling pathway is a major trend. In this thesis using the Populus genome fragment insert BIBAC library pCLD04541 plasmid construction, transformation of wild-type Arabidopsis by the floral dip method (Floral-dip), by screening and analysis the transformation plants that growth specifically compared with wild type, aimed at improving the Populus euphratica translational genomics research platform, further more screening Populus specifically trait gene (cluster). The main results are as follows:(1) Using 102 library clones, a total of 1320 Arabidopsis thaliana were transformed. And 225 transformed plants were got when 34 special strains were detected, among which there were 6 strains with observable traits. Because of abortion occurred and ultmately got five clones that can pass the gene to the next generation. The observable characters mainly concentrated in the plant stem and inflorescence development, lateral steim tiller formation and seed formation.(2) The plant morphology data were collected and analyzed by mathematical statistics. Morphological changes in the main:smaller, sessile, glabrous, main stem or deletion, inflorescence disappeared pods and variable short or abortion, fruit pods hyperplasia, an increase in the number, seed size, weight gain, and so on.(3) Found 2 cases of dwarf and abortive type in transformed plant; 1 case of pods shortened and seed number plummeted; 3 cases of rapid development, plant high and large and seed weight increase. And the reasons are analyzed. The results show that the 68A1G5,68B1E2 strains grow obvious dwarf compared with wild type, as the inflorescence disappeared and emergence of the different degree of abortion; 68A1E3 strain shows developmental abnormalities, with pods was significantly shortened and seed number plummeted. The 68A1B2,78B1B9,78B2A6 three strains grow obvious advantages than wild type, and have different degree appearance of branch increase, with the hyperplasia of the pod and seed weight gain. Under the same conditions, The 68A1B2,78B1B9,78B2A6 types growth development faster. We speculated that the large fragment of Populus euphratica gene have been successful integrated in Arabidopsis thaliana and express, that lead to the exhibited characters.