| Red blood cells has an important physiological function in human body, which mainly responsible for transport of oxygen and carbon dioxide in the body to ensure daily metabolism. Dyserythropoietic will cause serious disease such as sickle cell anemia and thalassemia, therefore it is very important to study the regulation mechanism in erythroid differentiation and development. Erythropoiesis is a complex and nuanced biological process that is regulated by transcription factors, chromatin conformation and non-coding RNAs. MiRNAs are widely expressed in eukaryotic organisms and play critical roles in the control of cellular differentiation, proliferation and apoptosis. It could improve our understanding of blood disorder to investigate the molecular mechanisms of miRNA in erythropoiesis.An in-depth analysis of genome wide miRNAs (miRNomes) in human embryonic stem cells (HESCs) and three erythroid cells at different developmental stages:HESC-derived (ESERs), fetal liver-derived (FLERs) and adult mobilized peripheral blood CD34+cells-derived (PBERs) was carried out in this study. We identified a total of 1029 known miRNAs and 12 novel miRNAs from four groups of data and found that there were significant changes during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage. We sorted out top 20 up/down differential expressed miRNAs that are most abundantly expressed in HESCs and ESERs respectively and predicted their target genes by integrating with our previous mRNA data. This comprised several significant biological functions, and pathways that related to erythroid differentiation. Furthermore, we focused on miR-185-5p which is the highest expressed differential miRNA in ESERs, experimental results indicated that miR-185-5p could promote ε-, γ- and β-globin synthesis and is potentially regulated by transcription factor GATA1. |
