| In order to explore the community structure characteristics and its driving factors of AMF in rhizosphere of S. pubescens in Daqingshan Mountain, Inner Mongolia, China, we sampled rhizosphere soil of S. pubescens in different sites, AMF spores were separated with wet-sieving and decanting technique and identified by the morphological methods.The results are as follows:1. The observation of the deyed root sample showed that S. pubescens easy to be infected and it is a typical arbuscular mycorrhizal plants.2. The driving factors of AMF community structure characteristics are seasonal and altitudinal variation and Available P, organic C, total N, pH and alkaline N in S. pubescens of Daqingshan Mountain, Inner Mongolia, China.3.46 AMF species belonging to 11 genera and 2 unidentified species were isolated from 81 sampling sites, the dominant species of 3 sites were F. mosseeae and R. intraradices, and Glomus has an absolute advantage in the 3 sites.4. There are 19 indicator species among all the species, and there are 11 indicator species in SMC,3 in XJG,5 in BSTG.5. The dominant genera of 3 seasons were Rhizophagus, Funneliformis and Glomus, and the dominant species of 3 seasons were R. intraradices, R. clarus and F. mosseeae. The spore density, the species abundance and the diversity index had significant differences between three seasons, and the maximum values of these three indicators were found in the July, but the minimums were detected at May, and the seasonal variation of AMF in rhizosphere of S. pubescens was consistent with plant phenology.6. The dominant genera of 3 slope positions were Rhizophagus, Funneliformis, Glomus and Claroideoglomus, and the dominant species of 3 slope positions were R. intraradices, R. clarus and F. mosseeae. The AMF diversity in rhizosphere of S. pubescens had significant differences between three slope positions, and the maximum values of these three indicators were found in the downslope, but the minimums were detected at upslope.7. The spore density, abundance and Shannon index and Simpson index were significantly positive correlation with pH, total N and organic C, while they were significantly negative correlation with alkaline N and available P. Available P, organic C, total N, pH and alkaline N are the main soil factors which affect the diversity of AMF. |
