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Screening Of Fermentation Bacteria And Utilization As Resources Of Eichhornia Crassipes

Posted on:2017-04-11Degree:MasterType:Thesis
Country:ChinaCandidate:L LiuFull Text:PDF
GTID:2180330485983425Subject:Environmental engineering
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Water hyacinth with scientific name of Eichhornia crassipes. is able to survive and grow in various kind of environment, under difference nutrient sources, temperature and pH values, such as rivers, lakes, cannals and all kind of backwater areas. Water hyacinth overgrows in eutrophic water, would gradually lead to water pollution, river block, ecological environment destruction and other diversity problems. Current disposure ways of dealing Water hyacinth are manual salvage and landfill. The aim of this paper is to explore utilization of Water hyacinth as resources.Four kinds of cellulose decomposing bacteria from the corrupted Water hyacinth were screened and purified. Bacteria C and B grew better to degraded cellulose.Water hyacinth strains and proportions of the vaccination of strains were studied the results showed that bacteria D is growing under pH 4 to 8. bacteria B and C grew better under pH 5 to 6 and they are all acidic bacteria. While under the same pH level, we found the bacterial colony sizes of bacteria B and C are larger than bacteria A/D. In the meanwhile, we conduct fermentation experiment of single and mixed bacteria and found that mixed fermentation can increase the degradation rate of strains, the best vaccination proportion ratio of bacteria B and C is 1 to 1.In the experiment of Water hyacinth fermentation, we found that conduct boiling and sterilization as pretreatment, fermentation broth dilution 2times,pH value keeps at 4, vaccination keeps atl.1ml, will get the best fermentation result. And the longer time the fermentation, the more dry cell weight get. e.g., take fermentation after 7days of cultivation, 1kg of Water Hyacinth can produce 3.5SCP under the optimized condition.On the basis of the above experiments, the process of producing single cell protein by using water hoist was designed, and the feasibility was analyzed.
Keywords/Search Tags:Eichhornia crassipes, Fermentation bacteria, Single cell protein, Resource utilization
PDF Full Text Request
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