Role Of PRMT5 In The Process Of Neuronal Migration

Neuronal migration is crucial for the construction of the nervous system. Positioning of neurons is the basic process of brain development. Disorder of this process can cause many types of brain diseases, and also contact with many neurodevelopmental disorders such as autism, mental retardation, epilepsy, and schizophrenia. Many genes have been proven to play an important role in neuronal migration. Protein arginine methyl transferases(PRMTS)regulate many important cellular processes by adding one or two methylated residues.PRMT5 is one of the member of PRMT family are distributed in the whole embryonic period.To study the effect of PRMT5 in neuronal migration, we constructed the PRMT5 plasmid vector, in utero electroporation was carried using the recombination vectors to discus the role of PRMT5 in neuronal migration. The results are as follows:1 Using in utero electroporation experiment and transfected PRMT1, 5, 7 and GFP into ventricle at E15.5, fixed after 5 days. Compared with the control group, PRMT1 and PRMT7 have no significant difference. But the PRMT5 positive neurons remained in IZ and VZ /SVZ, only a small portion reaches the MZ.2 To observe the location of PRMT5 and the change of neuronal morphology, we transfect PRMT5-MYC with GFP. Compared with the GFP control group, the leading processes of experiment group were significant growth. In CP and IZ, PRMT5 was appeared spotty and rod. When neuron arrived at MZ, PRMT5 was mainly appeared spotty.3 MEP50 is an important co-factor of PRMT5, but it had no effected on neuron migration when transfected lonely. When MEP50 transfected with PRMT5, the inhibition of neuron migration by PRMT5 was saved.4 MEP50 and PRMT5 transfected together at E15.5, followed by fixation at E18.5.Compared with PRMT5 group, the leading process returned to normal. PRMT5 was evenly distributed in cytoplasmic.In summary. PRMT5 inhibit the neuronal migration, over-expression of PRMT5 can inhibit neuronal migration and have special location, the leading process of positive cells are significant increase. MEP50 can save the inhibition of neuronal migration by PRMT5 and change the location of PRMT5, the leading process of neuron returned to normal.