UGPase Gene Cloned And Molecular Cloning Of MYB Gene Family And Temperature Stress Analysis In Dendrobium Candidum Wall.ex Lindl

UGPase is one of plant growth and sugar metabolic pathways important, kinase and plays a crucial role in sugar accumulation of Dendrobium candidum growth. MYB transcription factor family is one of the most important gene families in plants, many of which have close relationship with the resistance of plants. So this experiment from the two aspects of research.Firstly, we use RACE technology cloned full-length Dendrobium UGPase 1 gene sequences, it has 3054 bp. The open reading frame length is 153Obp, which encodes 510 amino acids, and the predicted protein sequence is 51.7 kDa, which is a hydrophilic protein. The analysis found that UGPasel protein only has some homology with Elaeis guineensis and Phoenix dacty lifera. We use bioinformatics software to predict the protein structure. Then using real-time q-PCR analysis of UGPasel in different tissues of Dendrobium, expression in different growth age. It was found that the amount of gene expression in roots and stems is much higher than that in leaves. Through reading and comparing analysis of Dendrobium officinale polysaccharide accumulation of literature, we found that the content of UGPasel gene may be proportional to the activity and the growth state of the sugar accumulation process.Secondly, a total of 12 sequences of the cloned MYB transcription factor family were obtained, which were in the range of 983-2320 bp. Encoding amino acid range of 203-410. We predict its subcellular localization.The phylogenetic tree analysis was established, and the similarity of amino acid sequence and the conserved domain of prediction sequence of the amino acid sequences were analyzed by MEME software.Analysis of the expression of DoMYB04 gene in the temperature stress by real-time quantitative PCR. We found that DoMYB04 gene expression obvious relationship between change rate and temperature, Dendrobium Plants can maintain the normal physiological state, there may be DoMYB04 gene expression changes. After the experiment in order to further understand and determine the relationship between DoMYB04 gene and temperature stress.