The Distribution Of Typical Antibiotics And Resistant Strains In The Sediment Environments Of Dalian

The study investigated the contentration and distribution of 14 sulfa-antibiotics and sul-resistant strains in the Dalian coastal 19 sampling points and seven typical sampling points including a number of estuaries, harbor, coastal city economy development of tourist areas, aquaculture and sewage treatment plants in sediment. The article further study the resistance strain distribution characteristic. Applicated HPLC-MS/MS to analysis the 14 kinds of sulfa-antibiotic concentration and using the EPA Method of 1604 in the 2002 promulgated can quickly detect the concentration of the E.coli in environmental. The research was based on the improved method screen the Escherichia coli(E.coli) and resistant E. coli (Re-E.coli). The percentage of Re-E.coli as the resistance level analyze the distribution characteristics of resistant strains in the sediment; Using Real-time PCR to quantitative the sulfa-resistance gene (sull, sul2, sul3) and intl and housekeeping functions genes (16s-RNA), calculating the relative copy number of the gene analyzed the distribution and the sources of resistance genes.The results showed that sulfonamides antibiotics widespread pollution in the coastal sediment of Dalian. The sulfonamides antibiotics detect range from ND to 699.62 ng/g. The level of resistant strains range from 0% to 33.2%. The summary of the domestics and abroad research, the concentration of Antibiotic resistance gene low residue in China than others. At the same time, the reseach showing the marine sediment have become antibiotic-resistant strains of the natural repository. Further study, the distribution characteristics of the resistant strains and resistance genes was typical with sampling points including the hospital waste (1, 4) and sewage treament plants (12,16) surrounding a large number of sampling points The sampling point (1,2,3,4,7,12,16) were higher anti-resistant strains gene than other sampling points. The preliminary study the distribution characteristics of resistant strains and resistance genes in the Dalian coastal which found sulfa-resistance gene distribut showing the feature of sul1>sul2>sul3. Meanwhile, the results explore the correlation between antibiotics and resistant strains on the using high concentrations antibiotic sampling points (1,2,3,4,7, 12,16). The positive correlation the total concentration of sulfa-antiboitic and antibiotic resistant strains (R2=0.836, p=0.05), showed the sulfa-antiboitic can induce the resistant strains under the high concentration. The sulfonamide resistance gene the relative copy number of sull and sul2 and intl were significant positive correlation (R2= 0.984, R2= 0.958), described the level of sulfa-resistance gene transfer in the presence of marine sediments.Conclusion as a result, there are the phenomenon of light pollution with sulfonamide and antibiotic-resistant strains in the coastal of Dalian and its distribution characteristics typical of marine functional zones were higher than other sampling points. As well as the highest levels in the surrounding hospitals and sewage treatment plants, the main source is the antibiotics induced and resistance gene horizontal transfer.