The Effects Of Environmental Factors On The Interaction Of Haloarchaea And Halovirus SNJ1

Haloarchaea and halovirus is one of the main microorganisms which live in the hypersaline environments. These microorgnisms play an important role in recycling the elements of biogeochemistry. However, until now, the systematic studies on the ecology of halovirus and the effects of varied salinity on virus-host systems are lacking. In this study,we explored the effects of environmental factors on the virus-host interaction through the haloarchaeam Natrinema sp. J7-2 and its virus SNJ1.The results were useful to understand the interaction between haoarchaea and their viruses and status of their ecology.This paper firstly studied the effects of main environmental factors(temperature, pH,NaCl concentration) on the infectivity of halovirus SNJ1 and the growth of its host Natrinema sp.J7-2. Then the effects of sodium chloride concentration on virus-host interaction was estimated. Finally fluorescence quantitative PCR was employed to assay the titer of halovirus SNJ1..The results are showed as follows:1. The tolerance level of low salinity on halovirus SNJ1 was lower than that of haloarchaeam Natrinema sp. J7-2. It showed that the latter one could existed in concentration of 12% sodium chloride, while the former could not. The activity of phage was subject to many factors, the survival rate of halovirus SNJ1 could maintained high within a certain time when NaCl concentration was 18%-30%, pH neutral or alkaline and temperature 4℃-45℃. Then the adsorption efficiency could keep the highest when NaCl concentration was 30%, pH neutral or alkaline and temperature 40℃.2. Halovirus SNJ1 acted as predators in the the high salt environment with NaCl concentration of 25%-30% to release progenic phages. While in low-salt environment with NaCl concentration of 18%, it could escape death by lysogenying its host cells.3. QPCR method shows good results, with a high salt environment monitoring phage dynamic potential.