Diversity And Community Structures Of Endophytes Of 4 Kinds Of Common Plants Growing In Mining Area Of Panzhihua

In order to investigate the diversity and community structure of the endophytes in different organs of common plants growing in mining area of Panzhihua, Sichuan Province. The research studied physical and chemical properties and heavy metal of habitat soil and heavy metals in plant organs of four plants which were Eleusine indica, CroftonWeed, Agave sisalana and Aptenia cordifolia.The denaturing gradient gel electrophoresis (DGGE) method was used to investigate the diversity and community structure of endophytic bacteria, fungi and actinomycetes.Through which to understand the distribution of endogenous microbes, even to provide a reference for environmental assessment The conclusions were as follows:(1) The highest diversity of endophytic bacteria of Eleusine indica was found in leaf, followed by stem and root. The highest diversity of endophytic bacteria of CroftonWeed was found in leaf, followed by stem and root. The highest diversity of endophytic bacteria of Agave sisalana was found in leaf, followed by root and stem. The highest diversity of endophytic bacteria of Aptenia cordifolia was found in root, followed by stem and leaf.The diversity of endophytic bacteria of Eleusine indica was the highest. The diversity of endophytic bacteria of Aptenia cordifolia was the least. The endophytic bacteria of the four plants growing in mining area of Panzhihua were phylogenetically categorized into Ochrobactrum, Luteibacter, Pseudomonas, Gemmatimonadetes, Pantoea, Thermus, Propionibacterium, Rhodococcus and so on.(2) The highest diversity of endophytic fungi of Eleusine indica was found in root, followed by stem and leaf. The diversity of endophytic fungi of root and stem of CroftonWeed was similar. The leaf was the smallest. The highest diversity of endophytic fungi of Agave sisalana was found in root, followed by stem and leaf. The highest diversity of endophytic fungi of Aptenia cordifolia was found in root, followed by stem and leaf. The diversity of endophytic fungi of root of Eleusine indica, Agave sisalana and Aptenia cordifolia was the highest. The endophytic fungi of the four plants growing in mining area of Panzhihua were phylogenetically categorized into Phoma, Peyronellaea, Alternaria,Chaunopycnis, Fusarium, Cladosporium, Aureobasidium, Aspergillus,Puccinia and so on.(3) The highest diversity of endophytic actinomycetes of Eleusine indica was found in stem, followed by leaf and root The highest diversity of endophytic actionmycetes of CroftonWeed was found in root, followed by leaf and stem. The highest diversity of endophytic actionmycetes of Agave sisalana was found in root, followed by leaf and stem. The highest diversity of endophytic bacteria of Aptenia cordifolia was found in stem, followed by leaf and root. The endophytic actionmycetes of the four plants growing in mining area of Panzhihua were phylogenetically categorized into Streptomyces, Nocardia, Pseudonocardia and Uncultured actinobacterium.(4) The results of cononical correspondence analysis (CCA) showed that the diversity of endophytic bacteria was negatively affected by copper (Cu), zinc (Zn), arsenic(As) and nickel (Ni), in which the Ni was the biggest. The diversity of entophytic bacteria was positively affected by chrome (Cr) and cadmium (Cd) (0.02 mg/kg≤Cr≤0.26 mg/kg,0.01 mg/kg≤Cd≤0.05 mg/kg). The diversity of endophytic fungi was positively affected by Cu, Pb, Cr, Zn and As (0.09 mg/kg≤Cu≤0.26 mg/kg,0.11 mg/kg≤Pb≤0.53 mg/kg,0.02 mg/kg5Crt0.26 mg/kg,0.28 mg/kg≤Zn≤2.28 mg/kg,0.00 mg/kg≤As≤0.09 mg/kg),in which the Cr was the biggest. The diversity of endophytic fungi.was negatively affected by Ni and Cd.The diversity of endophytic actionmycetes was positively affected by Pb (0.11 mg/kg≤Pb≤0.53 mg/kg).The diversity of endophytic actionmycetes.was negatively affected by Cr, Cu, Cd, As and Ni.