Inward Rectifier Potassium Channels And PIP₂ Binding Site Correlation Studies

With the rapid development of life science, the human genome has been gradually deciphered, the map of map will appear in front of the us, using genetic manufacturing drug treatment more difficult disease is no longer a yearning.With the development of computer science, bioinformatics also was born. Among them, the use of bioinformatics research on ion channels, appear as an important research direction in the line of sight of people.Kir(Inwardly Rectifying K Channel) is one of the widely distributed in all kinds of organization potassium channels, because of its voltage current relationship of inward rectifier spy the name. It is mainly distributed in the centrifuge, skeletal muscle, smooth muscle, It acts to maintain the cell resting potential, the regulation of vascular smooth muscle contraction and relaxation functions in the organism.Inward rectifier potassium channel function depends on the PIP2(phosphatidylinositol(4,5) bisphosphate). PIP2 Accounting for only less than 1% of the membrane phospholipids, but its function is very complex Currently, the research for the binding sites main have Molecular dynamics simulations and crystal structure analysis. Therefore, the use of amino acid sequence analysis revealed law for binding sites at positions predicted binding sites in the sequence has a more important practical significance.in order to study Kir and PIP2 binding site prediction model,we need to build a database that contains all Kir channels, SWISS-PROT database for screening, and selected seven Kir subfamily(Kir1-Kir7) 16 members and experts sequences, totaling 64. Each have an average sequence length greater than 350 amino acid residues. In order to predict the binding site, the need to integrate on the physicochemical properties of amino acids.It is broadly divided into amino acids chargeability amino acid pH, acids hydrophilicity, secondary structure propensity of amino acids, free energy value. And binding sites of amino acids and amino acid binding site of the laws surrounding the use of sequence alignment and statistical analysis methods.By the analysis of Kir and PIP2 binding sites modeling get the following conclusions. First, Kir has built a database sequence alignment to the literature have been measured binding sites of Kir channel as a reference, find binding sites and binding sites around the highly conserved amino acid type, and the formation of amino acids surrounding alkaline a basic group.This paper also obtained and analyzed Kir and PIP2 binding sites spatially continuous, discontinuous in sequence, divided into three regions, and through the analysis of experimentally derived binding site sequences within the threshold region measured Kir channel binding sites was consistent, and applicable to other Kir channels.