| The Ebinur Lake Wetland is the most typical wetland desert ecosystem in temperate arid zone in Northern Xinjiang. It is of vital importance in maintaining the eco-balance and moderating the climate in Northern Xinjiang. This research studied the physical and chemical properties of rhizosphere and non-rhizosphere soil of Halocnemum strobilaceum, Reed and Salicornia in different seasons and built the rhizosphere ammonia oxidizing bacteria clone library of the three plants in summer to reveal the differences of rhizosphere and non-rhizosphere soil properties of the three plants and the diversity of ammonia oxidizing bacteria community in the rhizophere of them. What’s more, the function of ecological instruction of ammonia oxidizing bacteria community’s diversity in the wetland ecosystem.In order to understand the health condition of the different plant rhizosphere from ebinur lake wetland, and provide the effectiveness scientific theoretical support for wetland restoration and sustainable development use, we collected the rhizosphere and non-rhizosphere soil of Halocnemum strobilaceum, Reed and Salicornia in April, July and October in 2013, and the soil moisture content, p H, ammonium nitrogen, Nitrate Nitrogen, electrical conductivity, organic matter, available nitrogen, available phosphorus, available potassium, soil eight ions content were measured quantificationally. The correlation in spatial and temporal variation scales of soil physicochemical indexes were analyzed. The results showed that the soil moisture content was lower on the whole and it had a very significant correlation with the sampling time. The p H was higher and it was alkaline soil. The electrical conductivity was at a relatively high level which showed that the salinity was high. It had a significant negative correlation with sampling plot. Overall, the ion content in the coverage area of three plants in the ebinur lake wetland soil is higher, which had correlation with p H, electrical conductivity. And the content of organic matter was relatively low on the whole. The average available nitrogen content was in the medium to low level and the average available phosphorus content was keeping in the medium to high level. The average available potassium content was in a higher condition which had significantly negative correlation with the sampling plot. The content of nitrate nitrogen and ammonium nitrogen had no significant correlation with sampling time and sampling sites, but were significantly positively related with water content and p H respectively. In addition, the soil quality of the Ebinur Lake Wetland was following the law of Halocnemum strobilaceum>Reed>Salicornia, rhizosphere>the rhizosphere and the law of the autumn>spring>summer on the whole. The results showed that the growth of plants may play a role in the improvement of soil fertility.In order to study the community diversity and its differences of rhizosphere soil amomonia-oxidizing bacteria, the Halocnemum strobilaceum, Reed and Salicornia in Ebinur Lake Wetland were investigated. The clone libraries of amo A gene were constructed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP), and phylogenetics were analyzed. To explore the community structure of rhizosphere amomonia-oxidizing bacteria, we combined rhizosphere physicochemical factors of the three plants. Phylogenetic analysis of the amo A gene fragments showed that all AOB sequnces from shrimp of three plants rhizosphere were affiliated with Nitrosomonas and Nitrosomonas-like phyla which both belong to the category of β-Proteobacteria, but Nitrosospira phyla was not discovered. The three AOB amo A gene liberaries include 9 OTUs, 12 OTUs and 7 OTUs respectively. The coverage of the three gene libraries was over 99% and they were of strong representativity. The richness index,chao1 index, ACE index and Shannon index of the three libraries was as followed. Reed rhizosphere AOB was much higher than Halocnemum strobilaceum rhizosphere AOB, and Salicornia rhizosphere AOB was the lowest. The community diversity of AOB in Reed was higher than that in Halocnemum strobilaceum, and the AOB in Salicornia is the lowest. The most bundant phyla of all plants rhizosphere AOB were different. This study provides a basis for understanding the community diversity and structure of rhizosphere soil amomonia-oxidizing bacteria in Ebinur Lake wetland.In order to investigate the ammonia oxidizing bacteria’s response to environmental factors from different plant rhizospheres, the SPSS 17.0 was used to analyse the relationship between the diversity and different communities of ammonia oxidizing bacteria and environment factors and the relationship among different ammonia oxidizing bacteria groups. The results showed that The environmental factors that affect the diversity of ammonia oxidizing bacteria community in the ebinur lake wetland were mainly nitrate nitrogen, available nitrogen and available phosphorus. The diversity ammonia oxidizing bacteria had positive correlation with the soil moisture content and organic matter, but had negative correlation with other environmental factors. The structure of ammonia oxidizing bacteria community was significantly correlated with soil salinity and organic matter content, and ammonia oxidizing bacteria in plant rhizosphere will influence each other in different groups. |
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