The Secondary Metabolites From An Endolichenic Fungus Periconia Sp.(No. 19-4-2-1)

Contents: The chemical investigation on an endolichenic fungus(Periconia sp., No. 19-4-2-1) was carried out to searching for compounds with novel structure or bioactivity. Methods: Compounds were isolated by ODS column chromatography, Sephadex LH-20 column chromatography, HPLC, etc. Then their structures were established by detailed spectroscopic analyses(UV, IR, MS, CD, 1D and 2D NMR), X-ray, and chemical reactions(such as Mo2(OAc)4 method). The cytotoxic and antimicrobial activities of compounds were also valuated. Results and Discussion: Sixteen new compounds [including eleven new tetracyclic fusicoccane diterpenoids(1?11), one new cadinane-type sesquiterpene(12), and four new polyketides(13?16)?, were isolated from the rice culture media of the strain Periconia sp.(No. 19-4-2-1), along with three known compounds. Among them, compounds 9?11 were the first cases of nitrogenous tetracyclic fusicoccanes. After bioassays, compounds 12?17 and 19 diaplayed moderate antimicrobial activity against Aspergillus niger, and showed weak activity against Candida albicans. This chemical investigation improves the level of understanding secondary metabolites from Periconia fungi. Furthermore, compounds 1?11 are new members of fusicoccane diterpenoids, which increases the structural diversity of fusicoccane diterpenoids.