Laser Micro-nano Hand’s Construction And Cells’ Phase Contrast Imaging

Since it was invented about twenty years ago, optical tweezers technology has been applied to many research fields of life. optical tweezers has become an important method in micro-nano manipulation. Scientific researchers carried out many abundant researches in the biological and sub-cellular fraction research field by using optical tweezers technology. In the nineties, DNA molecular dynamics, molecular motors and cell membrane dynamics has been researched by using optical tweezers. Quantitative phase imaging is an emerging microscopic imaging technique in recent years. It not only can see the transparent cells, but also provide the axial optical path difference resolution reaching nano-meter order. Therefore, combining quantitative phase imaging technology with optical tweezers technology to carry out cell study has strong practical significance.In this study, we combined the holographic optical tweezers system with the Olympus phase contrast microscope, the constructed system was used to capture the polystyrene micro-spheres and the human red blood cells, and do phase contrast imaging. By this preliminary study about quantitative phase imaging of SLIM, it will lay the foundation for the cell’s thorough research in the future. In this study, diffraction method was used to realize the green light gamma correction of the pure phase SLM. After the correction, the SLM can accurately modulate the phase of green light. We also completed the 633nm laser Gamma correction of the pure phase SLM by using double-hole interference method. and the system can accurately complete phase modulation of laser.In this research we use SLM to generate a double optical tweezers which light intensity can be adjusted, the double optical tweezers was used to capture the polystyrene micro-spheres and the human red blood cells. Finally, we achieved SLIM imaging based on the optical path of optical tweezers.