| Red-crowned Crane (Grus japonensis), first class of protected birds, was included in the World Conservation Union (IUCN) Red Data Book, levels are EN, and their conservation has received widespread attention around the world. Wild red-crowned crane is mainly distributed in China-the East Asia-Australia line, the current number of the eastern flyway subpopulation has been stable or slightly increasing, while the number of the western flyway subpopulation has sharply declined in recent years. So now conservation and artificial breeding of red-crowned crane is more important. Shenyang Forest Zoo established the Shenyang National Crane Artificial Breeding Research Base in 1990, which is one of the most successful Units in breeding work of red-crowned crane. Today, the red-crowned cranes in Shenyang are in large-scale, which occasionally output to the other units. In order to promote the healthy development of the population, the population’s genetic diversity and relationship were studied in this paper.In this paper, the genetic diversity of the core red-crowned cranes (Grus japonensis) in Shenyang Forest Zoo was analyzed by 14 SSR loci, with 9 highly polymorphic loci and 5 moderately polymorphic loci. The results showed that there were 99 alleles, the average alleles were 7.07, the average effective alleles were 3.0007, the average expected heterozygosity was 0.6017, the average polymorphic information content was 0.5620, showed middle level of genetic diversity. Hardy-Weinberg equiliberum (HWE) test showed that 7 SSR loci were in accord with the HWE and 7 loci were not in HWE, the entire group were not in HWE; more than half of the locus’s fixation index is positive, while the average expected heterozygosity was greater than the average observed heterozygosity, indicating that there may be closer relationships in some individuals; Genetic relationship analysis of species of cranes showed that the further of genetic relationship of the sexes more likely to be paired. |
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