Epigenetic Control Of AtPOE1Family Gene Expression

Pollen Ole e1domain-containing proteins were initially identified as major allergens. recent studies have suggested that these proteins function as developmental regulators in in many other plant tissues.However, there never have some systematic research on Pollen Ole e1domain in arabidopsis. In this study, we used a member of Arabidopsis thaliana Pollen Ole e1family (AtPOE1)--AtPOEl;26as a model, researching it’s function and the relationship with epigenetic control to construct the base of knowledge of the whole Pollen Ole e1family in arabidopsis by bioinformatics, molecular biology, cytology and Chromatin Immunoprecipitation (ChIP).The result we got are following:1. analyzed the whole Arabidopsis genome and identified at least28genes encoding proteins with a Pollen Ole e1domain, based on TAIR (http://www.arabidopsis.org/) annotations and These genes were classified into the AtPOEl family by bioinformatics.2. performed Reverse Transcription-polymerase Chain Reaction (RT-PCR) using multiple tissues from14-day-old and30-day-old seedlings of wild-type plants to understand the transcription profiles of AtPOE1-family genes. We found Most of the AtPOEl family genes showed specific transcription patterns.3.Constructed pAtPOE1;26:GUS plasmid and transformed into arabidopsis, we obtained20transgenic lines. GUS staining was only detectable in the root by using T2seedling.4. Constructed35S:YFP-AtPOE1;26plasmid and This construct was introduced into tobacco leaf cells. The Yellow Fluorescent Protein (YFP) signal was observed in both the cytoplasm and nucleus.5. through quantitative Reverse Transcription-polymerase Chain Reaction (qRT-PCR) compared the transcript levels of AtPOEl;26between the PRC2mutant clf-50and the wild-type. In both14-day-old rosette leaves and whole seedlings, the expression level of AtPOE1;26was significantly higher in clf-50than in the wild-type plants. Next, we performed a ChIP assay to detect the H3K27me3level at the AtPOEl;26locus in clf-50and the wild-type. The results showed that the H3K27me3level at the AtPOE1;26locus was significantly lower in clf-50than in the wild-type.