Cluster Analysis Of The Karyotype Resemblance-Near Coefficient Of Elaeagnus In Xinjiang

This article applies the method of karyotype analysis of Xinjiang region failed Elaeagnus taxonomy. In xinjiangAltay,Kashi,Turpan,Hami and Yli five regional sampling investigation a total of 57 strains. culture、chromosome and mapping. Crashing to Elaeagnus chromosome.karyotype parameters than observation.Comparative analysis the Xinjiang Elaeagnus failed relationships and evolution of genus relation. The main results were as follows:(1) For example,HM34 number.chromosome karyotype formula:2n=2x=28=8m (2SAT)+12sm+8st. According to carve of karyotype analysis methods can calculate that the longest and the shortest chromosome length ratio is 2.644,between 2:1-4:1,arm ratio of greater than 2:1 chromosome number accounted for 64.287% of all the percentage of the chromosome number of chromosome type belongs to the type "B".Karyotype asymmetry coefficient (As.K%) was 68.659%,the no.l for satellited chromosomes.(2) For example, YL2 number.the chromosome karyotype formula:2n=2x=28=12m+10sm+6st. According to carve of karyotype analysis methods can calculate that the longest and the shortest chromosome length ratio 5.364,greater than 4:l,arm ratio of greater than 2:1 accounting for 50.000% of all the percentage of the chromosome number of chromosome number, Its chromosomes type is "2 c".(3) Based on the analysis of karyotype seems almost coefficient,57 Elaeagnus plant a total of 14 pairs of chromosomes, chromosome numbers were:2n=14,diploid plants. Chromosome relative length differences, each type of genome often three to four sizes chromosomes. Average arm ratio between 1.746-1.511, the range of variation karyotype asymmetry coefficient is small, all of about 68 percent, one of the few plants have with the body, and most satellite is located on chromosome metacentric longest ratio as well as the range of variation karyotype asymmetry index of chromosome to the shortest are small, basic in 67%.(4) The use of cluster analysis software like karyotype near coefficients calculated 57 kinds Elaeagnus plants karyotype resemblance-near coefficients, which, YL10 and YL16 karyotype resemblance-near coefficient maximum (0.9973), the closest relationship, ALT4 and KS8 karyotype resemblance-near coefficient minimum (0.0249), the farthest genetic relationship.(5) The use of similar recent factor clustering method average (UPGMA) cluster can be 57 Oleaster plants are divided into seven categories. According chromosome type can be divided into two kinds, which 1、2、6、7 class as a karyotype is "2C",3、4、5 class as a karyotype is "3B"(6) By comparison with the results of Shenxiu Zhai found that 1、2、6、7 category is E. oxycarpa,3、 4、5 class is a E. moorcrofiii.In addition HM39 phenotypic traits measured as E. oxycarpa Schlecht. and by karyotype analysis was divided into E. moorcrofiii; HM35 measured as E. moorcrofiii in phenotypic traits, and by karyotype analysis was divided into E. oxycarpa.Two studies for classification under Elaeagnus species is basically consistent, indicating classification under the species, the use of the results of this study support karyotype analysis classification Shenxiu Zhai repair morphological classification.(7) By comparison with Panpan Wang studies found that 1、2、6、7 category is E. oxycarpa,3、4、 5 class is E. moorcrofiii.Because TLF5, YL14, YL12, HM35, HM36 this five plants in King Panpan study is divided into Elaeagnus, but karyotype study is divided into E. oxycarpa, no Elaeagnus presence of this genus. It shows that the classification under the species, the results of this study using karyotyping classification does not fully support the results of its morphological classification.(8) Planted grade classification, results of this study are divided into seven categories, which planted the classification did not correspond, indicate that under the species classification, the classification results of this study do not support them two human classification.(9) The karyotype asymmetry coefficient can know,1,2,6,7 class karyotype asymmetry coefficients were 65.83%,66.31%,65.89%,66.74%, while Class 3,4,5 karyotype asymmetry coefficients were 66.27%, 68.53%,66.49%.According to evolutionary nuclear performance by an asymmetric to symmetric,can be analyzed to obtain, E. oxycarpa is relatively E. moorcrofiii.belonging to more primitive species.