Analysis Of Cold Related Traits In Transgenic Rice Lines Of C2H2Zinc Finger Protein Family

C2H2transcription factor is one of the main member in zinc finger proteinfamily and that plays an important role in plant growth, development and abioticstresses. In this study, we used Gateway technology cloned71C2H2zinc finger genesfrom the diverse tissue in Nipponbare (Oryza sativa L. cv. Nipponbare) and attachedthem to the over-expression vector. We transferred the vectors into rice (Oryza.sativaL. cv. Kitaake) callus with agrobacterium mediated transformation method andobtained those transgenic plants.The main results were summarized as follows:1. Cloning and transformation of C2H2zinc finger genesThe japonica rice variety, Nipponbare, was used as the material. C2H2zincfinger genes had been cloned and constructed to the transcription activate expressionvector (VP64-pCambia1301-UbiN) and the transcription inhibit expression vector(EAR-pCambia1301-UbiN) by GatewayTM technology, respectively. We transferredthe vectors into japonica rice variety Kitaake using agrobacterium mediatedtransformation method. A total of335T1transgenic lines were gained in71C2H2zinc finger protein transcription factor family genes.2. The selection of excellent lines using T1transgenic rice linesAccording to the results of yield performance and heading stage,38genes wereselected in T1lines, and a total of105lines were used as sub-sequent test.3. Significance analysis of agronomic traits in T2transgenic rice linesA total of38genes were used significance analysis for7agronomic traitsbetween transgenic lines and wild type. A total of20genes showed significantdifferences on agnomic traits between transgenic lines and wild type in Changchun, and29genes showed significant differences in low temperature conditions. There areselected4cold tolerance genes (C2H2-10，C2H2-12，C2H2-44，C2H2-69) and3cold sensitive genes(C2H2-30、 C2H2-37、 C2H2-91) with low temperaturescreening.