The Regeneration Research Of The Silicon Substrate Membrane Adsorption Ability And A New Technology Development In The Process Of The Dye Marker Protein

The21st century is the century of biological science,significant changes havetaken place in biological science.The establishment of the theory of evolution havegreat influence on biology and other related discipline.The discovery of law ofinheritance have pointed out the direction of the development of genetics.The theoryof double helix structure of DNA and the accomplishment of Human GenomeProject mean that the development of biology are tending to internationalization.Generally speaking,the development of modern bioscience is mainly reflected inmicro and macro:The exploration of bioscience has been developed from cellularlevel to molecular level in micro level;The development of ecology has played anmore and more important role in solving the matter of global source andenvironment.As we all know,The exploration and development of bioscience can not doanything without the the support of the strong economy ranging from nationallaboratory to basic biology laboratory.In order to lower the cost,this paper aims athow to save cost in the extraction of nucleic acid and the stain of protein.Extraction of nucleic acid:the repeatability of adsorption column in TIAN pureMini Plasmid Kit had been explored in aspect of different solution、retention time、pH、ionic concentration and temperature of immersing adsorption column.The resultshows that:the adsorption column could effectively regain adsorption capacity aftersome treatment.The optimum condition is that:pH=2.04、4℃、0.1Mphosphate-buffered saline,in this condition,it can be conserved for6days.The abilityof adsorption can be stronger after the adsorption column is treated.Stain of protein:the protein sample is stained by reactive dyes,analysis the dyeingeffect by SDS polyacrylamide gel electrophoresis,the result shows that:reactivebrilliant blue has good effect on staining protein;The best stain condition of reactivebrilliant blue is:50℃water bath,stain for10minutes,the time of staining has lessinfluence on dyeing effect which mainly depends on the temperature.FORexample,the trypsin can not be stained by reactive brilliant blue.Single band can beobtained after dialysis treatment,pre-stain marker can be obtained by several protein.Through the experiment research,the repeatability of adsorption column in Mini Plasmid Kit and the possibility of making pre-stain protein marker in laboratory hasbeen studied.The ability of adsorption could be recovered,and the stain of protein isstill to be studied about involved protein and dyes.