Screening And Functional Identification Of Refractory Organics Degrading Bacterias In Deepsea

Refractory organics have been considered as major pollution source nowadays,and its excessive existence leads to damage of the ecological environment because thelimitation of self-cleaning capacity. The thesis choose feather, acetaldehyde, chitinand cypermethrin as representative, to isolate bacteria from deep-sea sediment ofseveral site, hoping to discover strain resources in deep-sea environment. The result isas follows.(1) Isolated from Nanhai Sea area, two Bacillus strains, designated Bacillus. sp.cha7and Bacillus. sp. cha8, were fully proved to be feather-degrading bacterias. Theinvestigation showed that, the feather degradation rate of Bacillus. sp. cha7andBacillus. sp. cha8were53.4%and35.4%, the protein concertration in1%feathermedia were1172.625μg/mL and177.45μg/mL, the amino acid concertration were285.38μg/mL and177.45μg/mL, respectively. Both of Bacillus. sp. cha7andBacillus. sp. cha8produced protein with molecular weight about60KDa and36KDa.The apparent characteristics and microscopic characteristics indicated that the strainswere able to cause erosion of feather. The amino acid detection implied that the aminoacids were variable due to different strains, but not the smash pretreatment of feather.(2) Isolated from North Pacific deep-sea sediment, an acetaldehyde-degradingPseudoalteromonas strain, designated NPYQ10A, was examined physiologically,chemotaxonomically and phylogenetically, and was proved to be novel species of thegenus Pseudoalteromonass together with a chitin-degrading bacteria isolated fromIndian Ocean in early stage (designated Pseudoalteromonas. sp. DXK012). Thedetermination of acetaldehyde degradation rate indicated that the strainPseudoalteromonas. sp. NPYQ10A was able to degrade acetaldehyde with100%rate,which implied its application potential.(3)14bacteria strains were isolated from North Pacific deep-sea sediment andproved to possess cypermethrin-tolerance characteristic, generlly above theconcertration of30ppm and9of them could able to tolerate the concertration with140ppm. To culture the strains with cypermethrin as sole carbon source, the result clearly showed that the strain could utilize cypermethrin, however, the common gaschromatography conditions was not fit for these strains.These creative investigations implied that a number of bacteria strains indeep-sea possessed unique degradation characteristics, and their degration ab ilitieswas also proved strong. In the practical application in the future, there is a hugepotential of these bacteria resources.