The Effect Of Histone Demethylase KDM2B To The Early Development Of Mouse Molar

Epithelial and mesenchymal tissue interact and induce with each other is the main regulatory mechanism during the tooth development. The regulatory mechanism function through the method that signaling molecules’ expression shows characteristics that depend on time and spatial. Researches about molecular regulation mechanism during tooth development are focused on the study of this four signaling pathways:BMP, FGF, HH and WNT signaling pathway. These studies help us understand the regulation of gene expression during the tooth development. With the development of epigenetic, research about epigenetic regulatory mechanisms in the process of organ development become a hot area. Histone demethylase is one of the important epigenetic modification enzymes, and involved in the regulation of gene expression by changing the state of histone methylation modification. In this study, we get a rudimentary knowledge of the exprssion change about histone demethylase during the early tooth development. And then, we detect the expression patterns of four histone demethylases Kdm2a, Kdm2b, Kdm3a and Kdm7a in the mouse molars development by in situ hybridization. The expression of this four genes can be detected in the bud stage, cap stage, and bell stage of tooth development. Finally, we interfere the expression of Kdm2b in mouse dental mesenchymal cells by a lentivirus-mediated RNAi. We find that depletion of Kdm2b during mouse molars development will result in tooth development dalay, and the dental mesenchymal cell maintain a high proliferation at the same time. This study has important tips significance in the research about histone demethlyases in the dental germ.