The Related Study On Biosynthetic Pathway Of Rubrolone

Tropolones are most produced by plant and fungi, with a small part by bacteria, which is a non-benzene aromatic substances with a seven-membered cyclic. And they have a wide range of biological activity. A red pigment rubrolone is one of tropolone derivatives containing nitrogen, which is a secondary metabolite produced by Streptomyces echinoruber sp.nov. Its molecular formula is C23H23NO8, and relative molecular mass is441. Its chemical structure is very complex and special as a complicated structure consisting of five ring and side chains having four hydroxyl groups. Rubrolone has a large polarity with soluble in water and methanol and insoluble in anhydrous n-butanol. The UV absorption peaks of rubrolone are216nm,275nm,421nm and523nm. The rubrolone has very low toxicity, so which can be used as a kind of food coloring.This study is mainly engaged in the research biosynthetic pathway of red pigment rubrolone. After doing a strain identified of Streptomyces echinoruber sp.nov in this experiment, we start to ferment the Streptomyces echinoruber sp.nov and to isolate and purify the metabolites. We also extract the Streptomyces’genome and construct the gene library, using primer of type Ⅱ polyketide syntheses gene as a probe to screen the genomic library. Then we get26cosmids which may contain a type II polyketide syntheses gene cluster. We want to mine the biological synthetic gene cluster of the red pigment rubrolone by taking heterogonous expression and gene knock-out technology. With the high-throughput sequencing of Streptomyces echinoruber genome, we study the relevant gene under the open reading frame (orf) prediction. The biosynthetic pathway of rubrolone will be presumed.