Cloning Of Panaxsaponin-glucosidase Gene From Absidia Sp.G8r

Panaxsaponin glucosidase produced by Absidia sp.G8r can hydrolyze panaxsaponin-Rb1to panaxsaponin-C-K that was have very high value in the medicine aspect. The panaxsaponin glucosidase express the strong particularity,which is never active for others.The results show that:Absidia sp.G8r bacteria producing ginsenoside-glucosidase with ginseng as the inducer, the induction effect is good.Use Catrimox-14TM Kit to extracted the complete total RNA. Additionally, primers were designed according to the homologous N terminal amino acids sequence of the panaxsaponin glucosidase from microorganisms Absidia sp. G8r.Carry an amino acids sequence design according to the N of the panaxsaponin glucosidase, set out from Total RNA of Absidia sp.G8r germtube, applied RACE technique, quickly expand to measure a preface to get cDNA3’ bitter end and cDNA5’ bitter end of sequence, made use of to expand cDNA that measure preface’s income5’ bitter end and cDNA3’bitter end of the sequence design lead and carry on two RT-PCR expanding and measure the whole sequenceses that the preface gets GluGF gene. The length of complete sequence is2149bp,3’UTR is114bp5’UTR is82bp,and the series of CDS length is1923bp.