Amylase And Lipase Immobilized On A Reticulated Polymer Containing Philip Ring Skeleton

Four kinds of philip ring skeleton reticulated polymer were synthesized. Characterization of their constructions and performance were tested. They were used to act as immobilize amylase and lipase respectively in order to investigate their performanc. Main contents were as follows:1.The copolymer of [4-aldehyde phenyl methacrylate]-EGMRA was used as carrier Ⅰ; while copolymer of [4-aldehyde phenyl methacrylate]-EGMRA-MMA was used as carrier Ⅱ. The-CHO of carrier and-NH2of amylase were ultilized to achieve the goal of immobilization of the amylase by Schiff reaction; It was indicated that the optimal immobilized time for immobilize amylase Ⅰ&Ⅱ were30min&10min respectively, while the optimal immobilized temperature were30℃and20℃. The activity of immobilized amylase were33.74mg.g-1.5min-1and23.85mg.g-1.5min-1respectively. Incubated at70℃for2h, activity of immobilized amylase were40%higher than the free amylase. And the stability for heat was better than the free amylase. Activity of immobilized amylase Ⅰ was68%while it was35%for immobilized amylase II. However the figure for free amylase was4%. Thermal stability and acid-base stability for immobilized amylase was superior to free amylase.16%of the amylase activity still existed after six times.2.The copolymer of [4-aldehyde phenyl methacrylate]-EDMA-MMA was used as carrier Ⅲ; while ammoniated polymer of Maleic rosin acrylic acid glycol ester-Methacrylic acid-MMA was used as carrier Ⅳ. The result show that the optimal immobilized time for immobilize lipase Ⅲ was30min; The optimal immobilized temperature was30℃, The optimal lipase supply of m(lipase): m(carrier) was0.14. The optimal cross-linking glutaraldehyde concentration for immobilize lipase Ⅳ was1%; The optimal cross-linking temperature was20℃; The optimal cross-linking time was2.5h; the optimal stir frequency was160r/min; The optimal lipase supply of m(carrier):m(lipase) was10; The immobilized lipase III&IV both have good thermal stability and acid-base stability. Incubated at80℃for2h, Activity of immobilized lipase Ⅲ was64%while it is43%for immobilized lipase IV. However the figure for free lipase was10%. Incubated at pH9.2for2h, activity of immobilized lipase Ⅲ was88%while it was97.5%for immobilized lipase IV. However the figure for free lipase was87%. Thermal stability and acid-base stability of immobilized lipase were superior to free lipase significantly.10%of the lipase activity existed after been used ten times for immobilized lipase III and98%for immobilized lipase Ⅳ. It was known that immobilized lipase Ⅳ had great repeatability.3. Catalytic activity of immobilized lipase Ⅳ to improve the esterification in a biological reactor. Results indicated that the optimal conversion rate of esterification reaction charging capacity was10g and the optimal feeding speed was lOmL/min, The esterification reaction conversion rate keeps9.5%after been used5.5h. It proves that the stability of immobilized lipase was increased.