The Effect Of Ubc9 On Neural Crest Cell Development Of Chicken Embryo

ObjectiveTo examine the effect of SUMO pathway on neural crest cell development of chicken embryo and to understand the function of Ubc9 in the neural crest cell, Providing theoretical basis for relative birth defects, treatment and prevention.Methods① Chicken embryos were collected, In situ hybridization and Immuno fluorescence were used to observe the expression of SUMO-conjugating enzyme-Ubc9 and early marker of neural crest. ②In the control group,5-mismatch Ubc9-control morpholino (5MM-Mo) were used to treat chick embryos;In the Ubc9-Mo group, sequence-specific Morpholinos(Mo) were used to reduce Ubc9 levels in chicken embryos, then the mRNA level of neural crest markers(Snail2, Sox9 and FoxD3) were observed.Results① Ubc9 were co-expressed with Pax7 in neural crest precursors of the neural fold at 4、6 and 8 stage of early chicken embryos. ② Compared to the control group,Ubc9-Mo treatment led to a reduce of mRNA level of neural crest markers,Snail2 (P<0.05), Sox9 (P<0.01) and FoxD3 (P<0.05)ConclusionSUMO-conjugating enzyme,Ubc9 expression started at neural fold at 4、6 and 8 stage of early chicken embryo, SUMO pathway is required for neural crest cell development of chicken embryo.