| SAGA(Spt-Ada-Gcn5 Acetyltransferase complex) is a multi-subunit and conservative transcription complex, which is composed of 19 subunits in fission yeast and regulates the transcription of 10% genes in vivo. In order to further research of SAGA, in this study, based on Hayashi and Matsuyama’s work, we constructed all integrated fluorescence strains of all SAGA subunits, and we analyzed subcellular fluorescence localization of all SAGA subunits. Microscopic data showed localization manners could be sorted by 4 types, suggesting that these SAGA subunits may have additional functions besides transcriptional regulation. Subunit Sgf73 is the bridge that connects deubiquitination module and other SAGA modules, lacking of sgf73+ not only significantly reduced nuclear fluorescence localization (NFL) of deubiquitination subunits Ubp8, Sgfll, Susl, but also affected NFL of acetylation subunits Gcn5, Sgf29, Nggl, and the core structure subunit Spt7. The impact indicates that Sgf73 is important to maintain enzymatic function and stabilization of SAGA. However, the other subunits’fluorescent localization in the absence of sg/73+ have no significant change, which indicates though these subunits exist in the same complex, the interactions between each other are not consistent. In addition, this study we also found when sgf73+ was deleted would also caused a cytokinesis defect, which is characterized by a multi-nucleus and multi-septum phenotype. In order to explore the cause of this defect, we carried out over-expressing ace2+ and mid2+ in Δsgf73, which are key genes involved in septum degradation. The result showed that ace2+ could not rescue the defect, and mid2+ could only partially compensate for the deficiency, suggesting that although sgf73+ may affected mid2+ expression or function, on the other hand, mid2+ could not completely covering the defects in Δsgf73, which means sgf73+ may also affect the expression or functions of other genes, so that jointly affecting the cytokinesis. |
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