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Analysis Of Murine Genomic DNA Methylation And Inheritance Effect On Carbon-ion Radiation Induced Bystander Organ

Posted on:2016-12-09Degree:MasterType:Thesis
Country:ChinaCandidate:Y L GaoFull Text:PDF
GTID:2180330461977007Subject:Biophysics
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To study the epigenetic changes on radiation-induced bystander effect and inheritable effect of bystander effect, the testis genomic DNA methylation changes of mice that got cranial radiation and variation characteristics in the offspring were examined. The male C57 mice(F0 generation) were selected as the experiment material.The mice were exposed to the dose of 200mGy carbon-ion radiation(energy 165MeV/n,the liner energy transfer 62.2KeV/μm,dose rate 0.5Gy/min)and were mated with unradiated female mice to give birth to the next generation(F1 generation). Intragroup mating of Fl generation yielded F2 generation. Methylation sensitive amplified polymorphism (MSAP) method was used to detect the information of testis genomic DNA methylation and demethylation.The study found that overall genomic DNA methylation polymorphism rates of the FO,F1 and F2 radiation group were 4.41%+0.67%(6 months after cranial radiation),6.36%+0.98% and 4.57%+0.23%,respectively, higher than that of the control group in every generation(p<0.0001). Analysis of testis genomic methylation and demethylation characteristics in each generation found that methylation trend was greater than demethylation trend in the changed genome of FO radiation generation while demethylation trend was greater than methylation trend in F1 and F2 radiation generation.These results demonstrated that cranial carbon-ion radiation of high energy could induce changes in genome methylation of mice reproductive organ.6 months after radiation,methylation trend was dominant. These changes could affect genome DNA methylation of reproductive organ in the offsprings via germ cells.The result of demethylation trend was dominant in testis genome DNA of progeny suggested radiation could change the stability of germ cell genome DNA by sperm.This kind of change reached the peak in the Fl generation and then droped in the F2 generation.The result showed that radiation could change genomic methylation of reproductive organ via bystander effect and cause instability of genital genome in progeny.
Keywords/Search Tags:Heavy-ion Radiation, Bystander Effect, Inheritance, DNA Methylation in Testis
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