Study On Lysozyme Detection And The Speciation Analysis Of Zn And Cr By Using Capillary Electrophoresis-inductively Coupled Plasma Mass Spectrometry

The main aim of this paper is to develop a novel method for the ultra-sensitive detection of lysozyme and different zinc and chrome species by using capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS), in hope of resolving the challenge of ultra-trace lysozyme detection in the clinical diagnose and the challenge of speciation analysis of trace zinc and chrome in the environment. The main contents includeing:(1), An ultra-sensitive method for the quantification of lysozyme based on CE-ICP-MS and the Gd3+ labeling was developed. The detection limit of the proposed method as low as 3.89 attomole. The proposed method has been successfully used to detect lysozyme in saliva samples with a RSD< %(n= 5) and a recovery of 91%-106%. The success of the present method provides a new potential for the biological assays and sensitive detection of low-abundant proteins.(2), A sensitive method for the simultaneous analysis of Cr+, Cr+and CrPic by CE-ICP-MS was developed. The detection limit of this method is as low as 0.4-0.8 ngCr/mL. The proposed method has been successfully used to detect the three different species of chromium compounds in health food samples with a RSD<6%(n = 5) and a recovery of 91%-106%. The success of the present method provides a new potential for the simultaneous analysis of both organic and inorganic chromium in heath foods which containing chromium.(3). A method for the simultaneous analysis of ziram and zineb was developed. The detection limit of the proposed method for ziram and zineb is 0.60 ngZn/mL and 1.0 ngZn/mL. respectively. The proposed method has been successfully used to detect the ziram and zineb in water samples with a RSD< 6%(n= 5) and a recovery of 95 %-108%. The success of the present method provides a new potential for the simultaneous and sensitive detection of metal pesticide in environmental sample.