Identification Of Reprogramming Intermediate Cells(KMEG-PRCS) And Secondary Reprogramming

Perviously, we isolated KMEG-prCs cell line during transcriptional factors transduction by Klf4, c-Myc, Glis1 and E-cadherin genes. Transcriptome analysis showed that the cells located at an intermediate status during reprogramming from somatic cells to iPSCs. It was similar to mouse embryonic stem cells in morphology, and proliferated on the feeder layer. However, it was unclear if KMEG-prCs derived pluripotency, and could be sequentially reprogrammed to iPSCs. In this study, the cell proliferation, expression of pluripotent genes, differentiation potency and DNA methylation of KMEG-prCs were analysized. The results showed that the double time of cell proliferation was similar among KMEG-prCs, ESCs and OSKM-iPSCs.In conclusion, KMEG-prCs kept partial pluripotency, and had obvious plasticity. It could be model to study mechanism of sequential reprogramming. In future, an optimized culture condition was used to reprogramme KMEG-prCs into full pluripotent cells, and it will be a model for study mechanism of somatic nuclear reprogramming.