The Study On The Electrical Characteristics Of The Excitable Cells Membrane Underlying A Temperature Gradient Field Induced By A Laser Irradiation

The application of laser in clinic becomes more attractive in recent years. It provides a new method for clinical diagnosis and therapy. In modern research, laser has been widely applied to internal medicine, surgery, gynecology, pediatrics, ophthalmology, otolaryngology, dentistry, dermatology, oncology, acupuncture, physiotherapy etc. Laser play an important role in biomedical research.Theme effect of laser interaction with biological tissue becomes an interesting subject to words in the versatile clinic applications. Photothermal effect was proposed to be a key factor in mediation when the infrared laser interaction with biological tissue. And this was employed at the tissue level. In recently year, the patch clamp technique was employed to record the electrical characteristic of the excitable cell membrane, and provided an effective method to evaluate the laser roles on the cell.In the study of ion channels, laser stimulation will change the activity of ion channels, will has an influence on ion channel opening and closing by changing the concentration of the ions around cell membrane and will affect the transportation of ion by changing the surrounding temperature, etc. To study the effect of laser on excitable cell, first of all, we need to study the distribution of transient temperature field, when study the laser irradiation on cells. Then to establish the poisson-boltzmann equation which describes the distribution of ion and analysis ion channel dynamics through the equation.This paper summarizes the development and application of laser in clinical medicine; introduces the various characteristics of biological membrane. When the laser irradiated on the target cells, refraction, reflection and scattering of light will happen on cell membrane. This paper introduces the transmission model of light in biological tissue, including classic Pennes equation, porous model and Weinbaum-Jiji model. This article stimulated the laser irradiated biological cells numerically using the finite element software and obtained the distribution of space temperature field of membrane. We compared it with patch clamp air electrode experiment, optimized the numerical model of laser irradiation on biological cells and get the temperature rise and temperature dissipation curve when the laser irradiation on biological cells. Afterwards we curve fitting out the temperature rise and temperature drop equation. According to the distribution of space temperature field of cell through numerical stimulation, we deduced the poisson-boltzmann equation under the space temperature field distribution related to the poisson-boltzmann equation under constant temperature which describes ion dynamics...