Genetic Analysis Of GRP7, RNP1and SIC1Mutant In Arabidopsis

A functional nucleo-cytoplasmic transport system is essential for plant development, signaling transduction and adaption to environment. In Arabidopsis, SICl encode a nuclear transport receptor belongs to the importinβ family. According to previous study, SIC1interact with two RNA binding proteins (RBPs) named RNP1and GRP7. However, the evidence for the interaction between SIC1and the two protein is limit to yeast two-hybrid test. Besides, it is not clear what changes would happen if SIC1fails to function normally. Here, as we have got the sic1mutant, we perform the analysis of genetic interaction between sic1and the GRP7, RNP1mutant to learn whether the function of the two protein contribute to the development of sic1mutant. By analyzing the development phenotype, we found no obvious similarity between sic1and the single or double mutant of GRP7and RNP1. However, the RNP1mutant shows some similarity to sicl on the response to some abiotic stress. The dwarf phenotype of sicl could be rescued to some extent when the RNPl mutation is introduced into sic1. In addition, overexpression of RNP1but not GRP7in sic1results in a more severe development defect. These results indicate that the phenotype of sic1is not simply come from the function defect of one or two protein. The detained RNP1in the cytoplasm caused by the defect nuclear transport may have a more profound influence in plant development, and this could be an important cause for sicl phenotype occurrence.