The Production Of Somatostatin Interneurons In The Olfactory Bulb Is Regulated By The Transcription Factor Sp8

Somatostatin (Som), one of the most concentrated neuropeptides in the brain, is highly expressed in the olfactory bulb (OB). However, the temporal profile by which OB somatostatin-expressing (Som+) interneurons are produced and the molecular mechanisms controlling this profile are totally unknown. Using the5-bromo-2’-deoxyuridine (BrdU) birth dating method, combined with immunostaining, we showed that the generation of Som+interneurons in the mouse and rat OB is confined to the later embryonic and earlier postnatal stages. Within the mouse OB, the production of Som+interneurons is maximal during late embryogenesis and decreases postnatally, whereas the generation of Som+interneurons is low during embryogenesis and increases postnatally in the rat OB. Interestingly, all the Som+interneurons in the mouse external plexiform layer (EPL) and the rat glomerular layer (GL) express the transcription factor Sp8. Genetic ablation of Sp8by cre/loxP-based recombination severely reduces the number of Som+interneurons in the EPL of the mouse OB. Taken together, these results suggest that Sp8is required for the normal production of Som+interneurons in the EPL of the mouse OB.