DNA Methylation Analysis

DNA methylation is an indispensable epigenetic modification required for regulat-ing the expression of mammalian genomes. Continued efforts are being made to un-ravel the methylation states genome-wide, featuring the Methyl-DNA immunoprecipi-tation(MeDIP) coupled with next-generation sequencing. Our method is a single-CpG-resolution, whole-genome methylation caller designed for MeDIP-seq data. It does not require external database for copy number adjustment. Furthermore, it effectively detects genomic regions potentially predisposed to oncogenesis through its prediction of states. The above suggests that our method makes a handy and reliable tool to generate genome-wide methylation profiles.