Bioinformation And Expression Patterns Analysis Of GH1β-glucosidases In Arabidopsis And Rice

Plants will encounter a variety of biotic and abiotic stress during development. High salinity leads to plants’ ion imbalance and hypertonic stress and makes destruction of water potential in plants and then influences the growth of the whole plants. Drought leads to water depletion of plants, which will destroy plants’bilayer structure of cell membrane, result in increasing membrane permeability, even destroy the integrity of the cell membrane and influence physiological metabolism of plant cells. Meanwhile, each plant has its own optimum growth temperature. Low temperature would lead to pollen sterility, which will cause the destruction of the cell membrane in serious cases. When plants encounter fungal infection, plant growth will be inhibited and serious cases can lead to plants die. And all of these factors are important reasons mat lead to decrease crop’s production.In plants, Glycoside Hydrolase Family1β-glucosidase is believed to play important roles in many diverse processes including defense, cell wall lignification, phytohormone activation and secondary metabolism. There are48GH1β-glucosidase genes in Arabidopsis and40genes in rice, which have seldom been studied in Arabidopsis and rice. We have little knowledge of the function of the genes, especially in terms of abiotic stress, which were rarely involved. This research studied the expression patterns of the47GH1β-glucosidase genes in Arabidopsis and37GH1genes in rice in the different tissues and adversity conditions, and analyzed evolutionary relationships and structure domain of the two family genes. The results are as follows:(1) Analyzing phylogenetic trees of the two gene families (AtBG and OsBG), we find that there are high degree genetic homology within the two family genes.(2) Domain analysis for the two gene families (AtBG and OsBG), we find that they both have three conserved motifs and each motif has highly conserved amino acids. (3) Under the different times of salt stress treatments, most of the AtBG family genes are regulated by the salt stress, but they have greater differences in expression patterns. At the same time point, genes of this family show up-regulated expression, down-regulated expression and microscale expression, most of the OsBG family genes couldn’t be induced to express and presented low expression levels under different processing time of salt stress.(4) Under different processing time of15%PEG, most of the AtBG family genes are regulated by the drought stress, but they have greater differences in expression patterns. At the same time point, genes of this family show up-regulated expression and down-regulated expression. Meanwhile, most of OsBG family genes presented low expression levels under different processing times of drought stress, but few of genes could be induced to express.(5) Few of the AtBG family genes could be regulated by cold stress under different processing time of4℃, among which the expression of AtBG7is significantly up-regulated and the expressions of BGLU40and BGLU11are significantly down-regulated when the cold treatment lasted for24hours. The expressions of OsBG family genes were very low under different processing time of4℃and only the expression of OsBG23is up-regulated when the cold treatment lasted for24hours.(6) Under different processing time of pathogen infection, few of OsBG family genes are regulated by pathogen. When the infection treatment lasted for24hours, the expressions of OsBG11and OsBG23were significantly up-regulated and the expressions of OsBG19and OsBG33were significantly down-regulated.(7) AtBG family genes are expressed in different parts of the Arbidopsis,but the expression quantities of some genes have a greater difference in the different tissues, some of which have high expression levels in flowers and seeds, and some of which expressed in stems and leaves but have low expression levels in roots. Also, OsBG family genes expressed in different parts of the rice too, among which OsBG7, OsBG8and OsBG38have more higher expression in flowers, OsBG14and OsBG25have more higher expression in seeds, OsBG5and OsBG22have more higher expression in leaves and OsBG28, OsBG29and OsBG34have more higher expression in roots.