Taxonomic Identification Of Nonomuraea Fuscirosea And Streptosporangium Shengliense And Study On Antimicrobial Activity Of All Isolates

Actinomycetes are extremely closely with human’s production and life, their metal are very variety, and they produce many useful substances. Many of their secondary m have been widely used in agricultural, industrial and clinical. The compounds from acti have characteristics with structural diversity, strong selectivity and great applicat Therefore, looking for new antibiotics from actinomycetes has been a major research d many scholars. In recent years, discovery and using of new antibiotics makes a great co to human health and medical career. So how can we effectively screen and filter new at In theory, directional filtering antibiotics can be done as the following aspects:selection chemical groups of antibiotic-producing bacteria; screening certain molecular mech antibiotic-producing bacteria; steering to the microbes that exist in nature and rarely st rare actinomycetes are an important source for isolating new compound and fin antibiotics; isolating, screening, identification of rare actinomycetes and filtering new c also have an important social significance and biological significance.In this study, we collected ten soil samples from different environment, includin under white pines, the soil of corn fields, the rhizosphere soil of rehmannia, mud of dry soil of Nanhu Park, soil from Qi panshan gutter and Yi tonghe gutter, forest soil outside th pharmaceutical factory and forest soil from Xinjiang. All soil samples were dried under th conditions for two weeks, then selectivly isolate of actinomycetes using dilution method media are GS and HV supplemented with cycloheximide50mg/l and nalidixic acid20total of113actinomycetes were isolated from ten soil samples, the plate method and cross were used respectively to determine the antibacterial activity of methanol extracts of ferm of113actinomycetes strains (the target strains including two kinds of bacteria and10fungi). Inhibition experiments obtained ten strains which antibacterial diameter against subtilis and micrococcus luteus were more than15mm, they were BS7, CF22, dht8, QPS1, CYQ5, GH6, GH8, XJ1and XJ8; eight strains at least significantly inhibited three kinds o they were CF3, CF7, CF22, CF26, CF29, BS3, QPS6and GH10. Antibacterial diameter agi fungi of one train (CF22) were more than15mm, so we repeated antibacterial activity expe HPLC, isolation and identification of compounds. We used multiphase method to identify strain NEAU-GH7and strain NEAU-dht8. First, we sequenced16S rRNA gene sequences of the two strains. Then, we did EzTaxon-e website online comparison and the results indicated that the two strains were affiliated to the genus Nonomuraea and Streptosporangium respectively. Strain NEAU-GH7’most related strain was S. longisporum DSM43180T (99.03%) and strain NEAU-dht8’most related strain was N. maheshkhaliensis16-5-14T (99.31%). Phylogenetic trees were constructed by the neighbor-joining and maximum-likelihood methods, the results showed that two strains all formed a phyletic line with their closest neighbour, an association that was supported by a high bootstrap value (>50%). Analyzing two strains’phenotypic characteristics (including cultural and morphology characteristics), physiological and biochemical characteristics (including the use of carbon and nitrogen sources, temperature range for growth, pH range for growth, NaCl tolerance range for growth, nitrate reduction, decomposition of cellulos, hydrolysis of aesculin, hydrolysis of starch, urease, esterase, catalase, liquefaction of gelatin, milk coagulation and milk peptonization, MR and VP) and chemotaxonomic characteristics (analyzing the type of phospholipids, menaquinones, amino acid of cell wall, whole cell hydrolysates and mycolic acids), DNA-DNA relatedness and (G+C) mol%confirmed that strain NEAU-GH7and NEAU-dht8represents a novel species of the genus Streptosporangium and Nonomuraea respectively, which the name Streptosporangium shengliense sp.nov and Nonomuraea fuscirosea sp. nov are proposed. The type strains are NEAU-GH71(=CGMCC4.7105T=DSM45881T) and NEAU-dht8T (CGMCC4.7104T=DSM45880T). The GenBank/EMBL/DDBJ accession numbers for the16S rRNA gene sequence of the two strains are KC513503and KC417350.