Analysis Of Atrazine Degrading Bacteria Arthrobacter Sp. DNS10Genome Bioinformatics

This study utilized the strain isolated in Heilongjiang province where has many years of atrazine application history in maize field soil (0-10cm) efficient atrazine degrading bacteria Arthrobacter sp.DNS10as the research object. Usie the Illumina Hiseq2000sequencing to get697Mb data, using SOAP denovo short sequence assembly software were assembled Clean Data, a total of5837130bp (GC content of52.06%gene prediction), genome size is6.03M. Genome analysis found that, the sample dns10genome contains5674genes, a total length of5053653bp, and the average length of891bp accounting for86.58%of the length of the genome. After assembly, analyzed by GC-Depth sequencing of GC bias, results show that the peak value is greater than50%. Genome analysis gene CDS sequence was obtained by gene KEGG, GO, COG, Nr and SwissProt database, the classification and annotation functions, different gene number, and classification of different database classification obtained, but are common interpretation of gene function. The metabolic pathways of gene types can be attributed to the carbohydrate metabolism, energy metabolism, lipid metabolism, nucleotide metabolism; this paper focuses on the analysis of the dominant gene of strain energy metabolism. The analysis shows that these metabolic enzyme function, dominant gene encoding are the same, but the gene encoding the same enzyme is slightly different; the same name although some gene annotation results, strain within the CDS sequence have several of the same gene, comparison showed differences in base composition is relatively large, poor homologous, this is the three consecutive base code for one amino acid, an amino acid can be composed of different bases, so the nucleotide homology search did not affect the amino acid coding, including its protein structure.Strain Arthrobacter sp.DNS10annotates to complete DNA replication path, shown on prokaryotic DNA replication proteins required for the main process, the strains were annotated, which makes this strain can be genetic, survival and stable in the environment. This strain is degrading bacteria, use atrazine as sole nitrogen source and therefore, nitrogen fixation genes not found in time to annotate genes work, this strain has more complete biological pathway of carbon fixation, in order to maintain the normal physiological function of strain. As a member of this strain of Arthrobacter, strictly chemoheterotrophic, have more abundant carbon source spectrum, in addition to glucose glycolysis/gluconeogenesis pathway, this strain also notes the amino sugars and nucleotides metabolism of glucose, galactose metabolism, starch and sucrose metabolism, fructose and mannose metabolism the relevant genes. Strain Arthrobacter sp.DNS10has a relatively complete three carboxylic acid cycle, oxidative phosphorylation, fatty acid metabolism and the synthesis and the phosphotransferase system, these pathways may provide their own large energy, maintain the normal physiological activities of the strains, and the process of reproduction, has important biological significance.Our study uses efficient atrazine degrading bacteria DNS10as the research object, degradation gene which contains trzN, atzB and atzC, have similar high and Arthrobacter aurescens TCI plasmid TC1At the same time by TA cloning success fully functioning sequence three gene。We through the online analysis software such as ORF finder, SOPMA, SWISS-MODEL and some other software, bioinformatics analysis, the main structural and functional analysis of three genes, and to understand their characteristics of three genes encoding enzymes of the superfamily and their.The experimental results show that there are more similar than99%major research model DNS10and several genes, suggesting that trzN, atzB, atzC three functional gene is very conservative, this phenomenon is also further evidence of gene with genetic in the original environment, and different parts of the world to different bacterial strains containing this view function gene the highly conserved. Through the comparison of the amino acid encoded three function with other strains, the amino acid of three functional genes encoding the gene sequence is highly conserved, which encoded a protein belonging to thefamily of TRZ/ATZ and metal hydrolase family, but at the same time, they have some differences in terms of protein family.