Study On The Ways And Means Of Regenerating Liver’s Cell Proliferation At The Transcriptome And Proteome Level

With a combination of transcriptomic and proteomic approaches, this study performed a comprehensi-ve bioinformatic analysis regarding the mechanismes of rat liver regeneration (RLR). The microarray studyshowed that the expression of total3712genes was significantly changed during RLR,2850of which wereup-regulated,816were down-regulated genes, and46were up/down-regulated. Protein analysis showedthat the expression of total2618protein was significantly changed, of which1518were up-regulatedproteins,411were down-regulated proteins, and239were up/down-regulated proteins. These genes andproteins were named as RLR related genes or proteins.2381kinds of RLR genes were unique to genome,775kinds of RLR proteins were unique to proteome and2383kinds of genes/proteins including1331kindsof RLR genes and1393kinds of RLR proteins were matched and related to liver regeneration at the mRNAand/or translational level.To understand the biological significant of these changes related to RLR viewed from the proliferationpoint，a large scale bioinformatic analysis was conducted based on the current information archived inseveral well established database and sophisticated statistics methodes. According to Gene Ontology (GO)bank,1009RLR genes and506RLR proteins are involved in cell proliferation. Compared those genes’expression change with those proteins’, found that561kinds of RLR genes which were unique to genome,89kinds of RLR proteins which were unique to proteome and744kinds of RLR genes/proteins werematched and related to liver regeneration at the mRNA and/or translational level. whereas606RLR genesand279RLR proteins are related to cell proliferation signaling pathways. The results of synergistic effect(Ep(t) value) analysis show that during rat liver regeneration, liver cell proliferation is mainly regulated byFGF、PDGF、INS、OSM、IL2and HRH3signaling pathways.9kinds of RLR genes which were uniqueto genome,3kinds of RLR proteins which were unique to proteome and23kinds of genes/proteins wererelated to those signaling pathways. Meanwhile, Analysed using network Principles and methods combinedwith Same Kind Extraction Method, found that the phospholipase Cγ2(phospholipase C gamma2, PLCG2)is the central node of those6signaling pathways. The expression of PLCG2was dually regulated bytranscription and translation, and played important role in RLR. In summary, this study applys the biological high-throughput detection combined withnetwork principle and method to analyse cell proliferation regulated by signaling pathways basing onexpression changes of gene and protein in rat liver regeneration, which lays a foundation to furtherexplore mechanism of liver regeneration.