Isolation, Genome Sequencing And Genomic Organization Of Novel Mycobacteriophage LiyA And The Activity Of The Product Encoded By Open Reading Frame Gp29/Gp30

LiyA is a new mycobacteriaphage isolated from Soil samples, It is dsDNA tailed phage and has a typical viral morphology with an isometric head and a long flexible tail, belongs to the Siphoviridae family. The LiyA genome is52175bp in length, contains92ORFs, and shows significant but distinct nucleotide sequence similarity with previous described mycobacteriophages L5.There are three tRNA encoding genes. Nucleotide sequence alignment shows LiyA belongs to A2family mycobacteriaphage.The complete sequences of80mycobacteriophage genomes show them collectively to encode101tRNAs, three tmRNAs. A statistical analysis predicts that approximately50%of the total number of proteinss are only present in in the mycobacteriophage population.The mycobacteriophages have provided a large number of information on the diversity of phages and identified a wealth of genes of lysis function.It is already known that, in that lysins, mycobacteriophages encounter a unusual problem and it is much different from phages of Gram-positive bacteria. The lysin from mycobacteriophage need to degrade the peptidoglycan and a mycolic acid-rich outer membrane, which covalently attached to the arabinogalactan-peptidoglycan complex. Mycobacteriophages accomplish this by producing two lysis enzymes—LysinA and LysinB. The functional roles of many mycobacteriophage ORFs are unknown, but Identified putative gene functions is essential to detecte mycobacteriophage evolution and function. Specially, the Lysins, encoded by most of phage genome, are well study as potential protein-antibiotics against a variety of pathogens, great tools for use in molecular biology and bio-probes towards developing detection technologie. So, it is significance to detecte of all kinds ORFs that encoded mycobacteriophage lysins, and a variety of motif in these Lysins. In this paper, we verified the encoding functional of gp29/gp30and dectecte the activity of degradation biofilm about the gp29and gp30of TM4, which is grouped into Cluster K with subdivision into subcluster K2on the basis of the nucleotide sequence similarities of TM4genome.