The Spermatozoa Ultrastructure And Spermatogenesis Of Two Species Of The Genus Gampsocleis

Gampsocleis gratiosa Brunner von Wattenwyl and Gampsocleis sedakovii Fiseher-Waldheim belong to Insecta, Orthoptera, Tettigoniidae, Gampsocleis. They are mainly distributed in North China and Northeast China. These two Gampsocleis are apt to collected in the field, and feed in the laboratory. The ultrastructure and the spermatogenesis of the sperm of G. gratiosa and G. sedakovii have been studied by paraffin section, DIC (differential interference contrast) and TEM (Transmission electron microscope) in this thesis. It would be theoretical and practical significance for reproductive physiology.The morphological changes of the sperm of G. gratiosa and G. sedakovii were ovserved by DIC. The spermatodesms, which is transferred from male to female by spermatophore, is considerably different between male and female even in the same species. In testis the sperm is single. In vasa deferentia, seminal vesicle and spermatophore, sperm are grouped together in bundles to form spermatodesms. There is a mucous cap that encloses the head of the spermatodesms. In female spermatheca, the spermatodesms is feather-shaped with a median axis.The ultrastructure of spermatozoon of G. gratiosa and G. sedakovii were examined by TEM. The mature sperm of two Gampsocleis are morphologically similar, containing three part: a head region, a neck region and a tail region. The acrosomal complex in the head region, which is composed of extra-acrosomal layer, acrosome, and perforatorium, lies laterally to the nucleus and embrances it partially. There is a pentalaminar organelle in the neck of the spermatozoon. Axoneme is typical 9+9+2 pattern. The two mitochondrial derivatives are partially crystallized. The sperm length of G. sedakovii is longer than that of G.. gratiosa. The cross-section diameter of the flagellum of G. sedakovii is larger than that of G. gratiosa. The included angle of the acrosomal complex of G. sedakovii is bigger than that of G.. gratiosa. The difference in spermatozoa structure of this two species of the genus Gampsocleis is not remarkable. The spermatogenesis of G. gratiosa and G. sedakovii , which were analysed on paraffin section, DIC and TEM, go through four stage: spermatogonia, spermatocytes, spermatids and mature spermatozoa. With the transmission electron microscopy, we observed that the electron density of the nucleuses of the spermatogonia is low, the cell junction is clear, and Golgi is plentiful. The characteristic of the primary spermatocytes is the nucleuses with low electron density, the blurred nuclear membrane and cell junction, prolific mitochondria and limited Golgi. The characteristic of the secondary spermatocytes is the nucleuses with electron-dense, clear cell junction, limited mitochondria and no Golgi. The spermatozoa collected from vasa deferentia have undergone a rearrangement. Some of them gathered into a bundle, whose head region was covered by a mucous cap. It was the same of the seminal veside and the spermatophore. When the spermatozoa entered into the spermatheca, the mucous caps of the spermatodesms have been degraded, and the head region of the spermatozoa grouped to the median axis to form feather-shaped spermatodesms.The feather-shaped spermatodesms in the spermatheca and the variation of the spermatodesms from male to female are characteristic in Tettigoniidae, the physiological significance of which needs further study.