The Discrimination Of Monosaccharides By Phenylboric Acid Derivatives Containing Amide Bond

Sugar which exists widely in nature is one of the three physical unit in living organisms. For organic life system suger has important significance. The smallest unit of sugar is monosaccharide ,it can be directly absorpted and utilizated. Glucose which is the typical representative of monosaccharide in the body, especially the levels in the body, plays an important role on it. So far the research on glucose levels draw people’s extensive concern. Examming the content of sugar is also an important challenge to medicine and industry. The monitor of the sugar content in microscopic system can be achieved by fluorescent sensors.The sugar containing multiple hydroxyls binds boric acid with a strong interaction. Therefore boric acid derivatives are often used to molecules recognition and cellular marker. When the fluorophores connects with sugar, it can construct fluorescent sensors for sugar molecules recognitionFluorescent sensors is made up of three main parts:(1) a receiving device (recognition and acceptance of the object, namely, receptors)(2) intermediates (connect the receiver with the report editor)(3) report device (produce the light signals). Overall, boric acid derivatives of sugar molecules recognition according to fluorescent chromophores types can be roughly divided into several categories:1. Anthracene–containing boronic acid derivatives2. Naphthene–based boronic acid derivatives3. Heterocyclic boric acid derivatives4. Boric acid derivatives containing pyrene ring5. viologen boric acid derivatives6. Other types of boric acid derivativesIndicator in the recognition of sugar molecules can make the fluorescence intensity of chromophores rising violently. Through the change of color, we can observe and analyze boric acid derivatives bonding situation intuitively. We research on boric acid derivatives and sugar molecules combination qualitatively and quantitatively by joining sugar or polyols.This paper by ARS - including amide bond boric acid derivatives - sugar molecules three component system to realize molecular recognition. It includes two big parts:For the first part I decide to use 9 boric acid derivatives containing amide bond which are not reported in literature , 5 for single boric acid compounds, 4 for double boric acid compounds to recognize 5 monosaccharide. In the pH=7.4 phosphate buffer solution , studing the changes of 9 benzene boric acid derivatives and glucose, fructose, sorbic sugar, mannose and galactose and so on five monose element, and the interaction by measuring the changing of compounds and their fluorescence spectra.The method of using indicator to join the sugar molecules identification system is very popular. My work is to analysis, calculate and compare the recognition effect of nine benzene boric acid derivatives,gain the related role constants.The second part is trying to recognize 3 kinds of sugar molecules in pH=7.4 phosphate buffer solution and in LDHs water system by 3-fluoro phenylboric acid. By measuring the fluorescence spectra of compound and sugars, to study their interaction. I use the indicator to construct sugar molecular recognition system. The results show us that: intercalated hydrotalcite system can well increase the recognition ability of 3-fluoro phenylboric acid to three kinds of sugar molecules . The recognition ability of glucose and galactose have a remarkable improvement.