Investigation On Three Kinds Of Novel Hydrogen Peroxide Electrochemical Biosensor

Hydrogen peroxide (H2O2) electrochemical biosensors have been widely used because of its simple fabrication, inherent high selectivity and sensitivity. In this thesis, we composed three kinds of hydrogen peroxide electrochemical biosensors using Au-Pt-PANI NPs or Fe3O4-PpPD NPs composite nanomaterials, which are used to investgate the electrochemistry behavior of Heme Oxygenase. The research has certain scientific significance and application value. The thesis consists of three chapters. The author's main contributions are summarized as follows:1,Overview the application of nanomaterials and redox proteins (enzymes) in H2O2 electrochemical biosensors which are from 116 references.2. Study the direct eletrochemistry of hemoglobin (HB) by CHIT-Au-Pt-PANI NPs and CHIT-Fe3O4-PpPD composite nanomaterial films and structured two novel electrochemical biosensors for the determination of H2O2. The results indicated that CHIT-Au-Pt-PANI NPs and CHIT-Fe3O4-PpPD composite nanomaterial films with good biocompatibility can not only accelerate the direct electron transfer of redox proteins (enzymes) but also improve the stabilities of the biosensors. It can be conclude that the present studies extended the application of nanomaterials in novel electrochemical biosensors.3. Fabracation of a novel H2O2 biosensor based on enzymatically induced the deposition of electroactive polyaniline (PANI) at the horseradish peroxide (HRP)/aligned single-wall carbon nanotubes (SWCNTs) modified Au electrode, and investigated its electrochemical behaviors. The electrochemical impedance spectroscopy of the biosensor confirmed that the formation of PANI on SWCNTs through the HRP catalytic reaction. In the biosensing system, a highly simple, sensitive and selective biosensor for the detection of the H2O2 was achieved in connection with the biocatalytic deposition of PANI. The above studies may provide a general platform for the construction of bioelectronic devices and the design of novel electrochemical biosensors with other bionano-systems.