The Fabrication Of Electrochemical Immunosensor Array

Electrochemical immunosensors which can detect the sample by the analysis of the antigen-antibody reaction are based on the immnunoaffinity. Immunoreaction processed on the surface of the sensor with good specific performance and high sensitivity. The immunosensor can analyze trace substance in a very short time, which is widely applied in the fields of clinical analysis, food analysis and environment analysis ect. Biosensors micro array can achieve synchronous analysis with different parameters, become one of the biomedicine analysis development.This paper has four chapters. The main research contents of each chapter are as follows:1. By the survery of past literatures, the theory of biosensor and immunosensor, and many methods of immobilizing the biomolecule were summarized. And also the applications and principles of the micro array electrochemical biosensors.2. Study two ways of self-assembled monolayers to immobile biomolecule: 1, A HCV electrochemical immunosensor was developed by assembling 3-mercaptopropionic acid (3-MPA) on gold electrode surface to binding the thionine and the HCV antibody. The CV voltamograms of the immunosenor displayed a couple of redox peaks current of the thionine. The peak current decreased after the HCV antigen reacted with the HCV antibody. While used in the clinical application, the results were in good agreement with the ELISA. 2, The immunosensor was prepared by co-immobilizing thionine and Hepatitis B surface antibody (HBsAb) on the gold electrode (GE) through covalently binding them to GE with a cysteamine/glutaraldehyde linkage. HbsAg (Hepatitis B surface antigen) was detected qualitatively and quantificationally by the peak current decrease percentage (△ Ip %) of the thionine. The CV voltamograms of the immunosenor displayed a couple of redox peaks current of the thionine. The peak current decreased after the HBsAg reacted with the HBsAb. The △ Ip % was proportional to the concentration of the HbsAg ranging from 0.85×10-2～5.12×10-2μg/ml and 0.32～1.28μg/mLμg/mL with the correlation coefficient of 0.981 5 and 0.990 6. While used in the clinical application of human serum, this method was in good agreement with the ELISA.