Design Of A Novel Porphyrin Colorimetric Array Sensor And Its Application In Biomolecules Detection

The detection of biomolecules and biosystems plays an important role in medical diagnosis. Currently, the large analytic apparatus are the common method for biomolecules and biosystems detection. Although the large analytic apparatus can ensure the detection accuracy, it brought us high cost and low detection efficiency, So they can not be widely used in medical diagnosis.In order to develop a new method for the rapid detection of biomolecules and biosystems, a novel colorimetric sensor array was designed and manufactured in the study. Then the colorimetric sensor array was used for the identification of amino acids, proteins and cell metabolism. Specifically, following work was completed in the paper.1. A colorimetric sensor array system was designed and fabricated, Concretely speaking, four part of works were completed: hardware design, software design, sensor array design and sensitive dyes'selection. Through hardware design, image acquisition before and after reaction can be done. Through software design, image processing and detection data visualization can be done. Through sensor array design and sensitive dyes'selection, a novel sensor array chip which was sensitive to biomolecules and biosystems was done.2. Important biomolecules and biosystems were detected by the colorimetric sensor array system.Amino acids, proteins and cell metabolism were identified by the colorimetric sensor array. The results showed that different amino acids can be easily distinguished by the colorimetric sensor array. It can even reflect the chemical property difference of the different amino acids. Then, different proteins can also be easily distinguished by the colorimetric sensor array. And it can also reflect the chemical property difference of the different proteins. At last, same cells cultured with different times and different cells cultured with same times can also be distinguished by the colorimetric sensor array.This study showed that the colorimetric sensor array system designed and fabricated here can be used for the rapid detection of important biomolecules and biosystems. Comparing whit large analytic apparatus, the colorimetric sensor array has the advantages of rapid detection, low-cost and high sensitive. It suggests that the colorimetric sensor array system may become an important method in medical diagnosis in the future.