The Application Research Of New Microbiosensors For Detecting Microorganism

The detection and identification of microbiology should involved in the area of analytical chemistry, life science environmental monitoring etc. In this thesis, three kinds of new microbial sensing methods were presented base on the response of electrochemical and SPQC sensor to the change, which related to some characteristics of microbial growth and metabolism, micro life activity. The detection is rapid, simple, and accurate. The main work of this thesis could be summarized as follows:1. A new method for the rapid identification and quantification of Pseudomonas aeruginosa using multichannel series piezoelectric quartz crystal (MSPQC) was proposed. The identification of P. aeruginosa was based on the development of acetamide broth, which can selective culture P. aeruginosa and performed perfectly in MSPQC. The quantitative detection of P. aeruginosa was based on the fact that the frequency detection time (FDT) detected by MSPQC in developed medium had a linear relationship with the logarithm of its initial concentration in the range of 10–108 colony_forming units (cfu) ml–1 (R= -0.984). The detection limit was 10 cfu ml–1.2. A new method for the rapid identification of Salmonella spp. Using multi- channel series piezoelectric quartz crystal (MSPQC) was proposed. The identification of Salmonella spp. was based on novobiocin and malachite green can inhibit the growth of other bacterial, but Salmonella spp can grow well in MSRV broth and produced CO₂, CO₂ was dissolved in KOH. During the process of growing Salmonella spp., the conductivity of the medium changed and was automatically monitored by MSPQC.3. A new method for detection of vesicular stomatitis virus(VSV) using an interdigital electrode modified by phosphatidylserine electrochemical sensor (PS-IDE-ECS)was proposed. In this work, interdigital electrode coated with phosphatidylserine was used as probe. The probe was directly connectes to electrochemical workstation. Phosphatidylserine as receptor can adsorpt VSV G protein, lead VSV infused into phosphatidylserine membrane, and holes formed on the phosphatidylserine membrane surface, in the constant voltage, resulting in transmembrane current, so as to achieve the purpose of detection.