Study On Optical Biological Sensor Based On ZnO

In this paper, based on the principle of surface plamas resonance, self-built the sensor system working at angular interrogation mode based on spectrometer. Investigate the sensitivity characteristic of different films as sense film with change of refractive index of ethanol solution. The experimental results proved that the sliver films as sense film system has the highest angular sensitivity, but has the worst stability. When the sliver thin film and the SiO2 thin film as sense organ, the system has the worst angular sensitivity, but has much better stability. And when the chromium thin film and the gold thin film as sense film, the system has the middle angular sensitivity, also has much better stability. According to these research results, used the chromium thin film and the gold thin film as sense film, a novel physical experiment based on spectrometer was developed.We grew the pure the ZnO thin film and the 1% Al-doped ZnO thin film on the Si (100) substrates by electron beam evaporation method. Investigate the crystalline structure and optical properties of the films with different growing condition. The result indicated that the film is strongly oriented to the c-axis of hexagonal structure and good crystalline when the substrate temperature is 200℃. The photoluminescence spectra of this sample exhibit two peaks, one is near band edge, and the other is the deep-level emission which due to oxygen and Zn defects. Then, there is no deep-level emission observed in Al-doped ZnO thin film grown on Si (100) substrate when the substrate temperature is 200℃.Sol-Gel self-assembled method was chosen to fabricate ZnO nanorods arrays on quartz glass substrates. It is clear that the sample post-annealing under 550℃exhibit highly preferred orientation with c-axis perpendicular to the substrate surface. The SEM images of the surface and cross-section of the films annealed at the different temperature show that the grain size uniform increased with increasing annealing temperature. However, the photoluminescence spectra of these samples also have strong deep-level emission.At last, investigate the Fluorescence Resonant Energy Transfer (FRET) based on the principle of evanescent wave and photoluminescence spectrometer. We chosen the blue fluorescent protein (BFP) as donor and green fluorescent protein (GFP) as acceptor studied the FRET between the BFP and GFP based on Al-doped ZnO thin film. The experimental results proved that the photoluminescence of Al-doped ZnO thin film maybe plays the role of excitation to stimulate the fluorescent donor molecule. Then the FRET occurs between BFP and GFP.