| Aim: to explore the changes of the proprioceptor amount and density of blood vessels in ACL remnant after its injure in rabbit,and provide theoretical basis for ACL reconstruction with preservation of remnant.Methods: 24 male New Zealand white rabbits were randomly divided into 6 groups(group A-F), 4 in each group. Group A was set as blank control group, the rest were the experimental groups. In the experimental group, the anterior cruciate ligament of the rabbit right hind limb was isolated from the femoral end, and the fracture model of the anterior cruciate ligament was established. Respectively after transection of 2, 4, 8,12,24 weeks the animals were sacrificed, and taken out the anterior cruciate ligament as exemplars. HE staining and immunohistochemical staining were performed to observe the type and number of proprioceptor, microvessel density.Result: From the experiment, we found that 4 weeks afrer ACL rupture, proprioceptor appear different degrees of degeneration and atrophy, reduced volume, color fades, irregular shape and internal structure of disorder and with the pass of time, the number of proprioceptors in ligament decreased. Rupture after 4 weeks, microvessel density was still at a relatively high level, after which showing a gradual downward trend. At 12-24 weeks, the microvessel density was almost the lowest, which was significantly lower than that of the normal anterior cruciate ligament.Conclusion: The amount of proprioceptors in the remnant of ruptured ACL was decreased with time going, and after 24 weeks, proprioceptor was no found. Rupture after 4 weeks, microvessel density was still at a relatively high level, after which showing a gradual downward trend. At 12-24 weeks, the microvessel density was almost the lowest, which was significantly lower than that of the normal anterior cruciate ligament. We suggeste that the ligament should be reconstructed as soon as possible after the ACL was broken. |
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