Effects Of Different Doses Of Fructose Intake On Glucose Metabolism In Rats During Pregnancy

Objective: By gavage administration with different doses of fructose water to pregnant rats,and observe rats blood glucose and blood lipid changes and embryo development, to explore the fructose intake on maternal glucose and lipid metabolism in rats and embryo development.Methods: After one week adaptive feeding,adult male and female SD rats were mated with a 2:1 ratio,vaginal smear were made on the morning of the next day, while sperm were found under a microscope records as gestation day 0, 48 pregnant SD rats were randomly divided into 4 groups, the blank group(BC group), normal dose of fructose group group(NF), high dose fructose group(HF group), super high dose fructose group(SHF) group, Separately gavage administration with 1ml tap water or 2.0g/kg, 5.0g/kg,8.0g/kg fructose water, on the 18 th day of gestation to implement oral glucose tolerance test(OGTT).Rats were sacrificed On the 20 th day.(1) the daily feed intake was recorded and weighed weekly;(2)OGTT examination were implemented on eighteenth pregnant days to evaluate glucose tolerance;(3)fasting blood glucose, insulin levels were detected, and insulin sensitivity index and secretion index were calculated to assess pancreatic islet function;(4) pancreatic tissue hematoxylin-eosin staining to observe the morphological changes.(5) get the fetuses while caesarean,and observe the growth condition,compare fetal body weight, the ratio of implantation, fetal death and fetal absorption.(6) detect the serum total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL), low density lipoprotein(LDL) levels;(7) specimens from liver tissue were weighed, hematoxylin-eosin staining was used to observe the changes of the organization;(8) enzyme-linked immunosorbant assay of inflammatory cytokines: tumor necrosis factor alpha(TNF- alpha) and interleukin-6(IL-6) level.Results: After 3 weeks gestation, increasing body weight of NF group, HF and SHF group was significantly higher than that of the BC group, the difference was statistically significant(P < 0.05);OGTT results showed, compared with the BC group, HF group sugar absorption peak appeared 15 min after glucose administration and was significantly higher than that in group BC and NF group(P < 0.05),SHF group take 30 min to come out the peak and take significantly longer time compared with the other groups; on the 20 th day of gestation, compared to the BC group, TC, TG, SHF group showed significantly higher fasting blood glucose, TC, TG, LDL than that of BC group, decreased insulin sensitivity and the degree of IR is more serious(P < 0.05). Liver pathological section observation showed that the BC group,NF group and HF group organization form had no obvious change, SHF group had a small amount of lipid vacuoles, slight fat deposition; pancreatic pathological observation showed that, in group BC and NF group, pancreatic islet cell morphology rules, normal size. In group HF and group SHF, there were different degrees of hypertrophy and hyperplasia. HF group stillbirth proportions were 9.1% was significantly higher than that in group BC group(P< 0.05). SHF group absorbed fetus, stillbirth proportions were 10.0% and 21.81% was significantly higher than that in BC group and SHF group fetal weight was significantly lower than that of the BC group and the control group(P< 0.05).Conclusion:1) High dose and high dose of fructose intake can lead to impaired grape tolerance and insulin resistance. 2) High dose and high dose of fructose intake can cause adverse pregnancy outcome. 3)High dose and high dose fructose intake induced abnormal pregnancy glucose metabolism and the increase in the level of inflammatory factors induced by fructose intake.