Expression And Significance Of S100A4 Protein And P53 Protein In Hypertrophic Cardiomyopathy Induced Myocardial Fibrosis

[Objective]Myocardial fibrosis is a hallmark of hypertrophic cardiomyopathy, S100A4 and P53 protein is closely related with the incidence of myocardial fibrosis. This experiment was to investigate the expression of S100A4 and P53 and their correlations with the myocardial collagen fibers in hypertrophic cardiomyopathy.[Methods] The HCM group was consisted of 10 myocardial tissue of patients undergoing ventricular septal myectomy for HCM at Fuwai Hospital, Beijing, China, from Jan 1,2013 to Apr 14,2014. 10 cases of traffic accidents were selected as control group respectively.Collagen volume fraction were observed and compared by Sirius Red F3B(SR) staining. The immunohistochemistry S-P and in situ hybridization technique was used to test the different expressions of S100A4,P53 proteins and their m RNA.Image-Pro Plus 6.0 software were used for quantitative analysis.The Student’s t test was used to prove the difference significance. A value of P<0.01 was considered statistically significant.[Results] Collagen volume fraction in HCM group was significantly higher than that in the control group(t=4.105,P<0.01).The expression of S100A4,P53 protein in cardiomyocytes of the HCM group were significantly higher than that of the control group(t=7.628,P<0.01)and S100A4 and P53 in HCM group are higher than that in control group at m RNA levels(t=3.464,P <0.01).[Conclusion] The increased contents of collagen fibers and the overexpression of S100A4 and P53 may play an important role in myocardial fibrosis of HCM, and they can be used as a new study direction for the HCM.Blockade of S100A4 may have therapeutic potential in hypertrophic cardiomyopathy by attenuating cardiac fibrosis.