Inhibitory Effect Of Selenium - Enriched Mycelia Crude Polysaccharides On CNE2 Nasopharyngeal Carcinoma Cells, Lymphocyte Proliferative Activity And Development Of Healthy Jelly

Currently, selenium and pharmacological studies of GLP have been gradually deepened, also With good clinical application. By pre-market research we found that the current has not been found to be Se-enriched mycelium as raw material health food market. Accordingly, the theory through more experiments to verify its safety and effectiveness, and one of the beneficial ingredients (such as selenium polysaccharide Ganoderma lucidum) extract accommodate large-scale industrial production, for the development of health food has enhanced the effectiveness of immunity reference has important significance and broad market prospects.Application of industrial production of liquid fermentation technology, selenium and organic Ganoderma together, developed a selenium-enriched Ganoderma; clarify the influence of Se Ganoderma lucidum polysaccharides on nasopharyngeal carcinoma cells and peripheral blood lymphocytes, with enhanced immunity efficacy results, the current selenium polysaccharide Ganoderma lucidum on the proliferation activity of nasopharyngeal carcinoma cells has not been reported.In this study, by using an industrial fermentors performed Se-enriched 500L fermentation of Ganoderma, and many times through the single factor experiment to determine the most suitable Se-enriched Ganoderma lucidum polysaccharides extraction conditions:neutral protease dosage of 1.5%(W/W), solid-liquid ratio of 1:30, the optimum reaction temperature is 50℃, pH value of 5, reaction time was 60min, added in an amount of 40% ethanol. And through a number of purification steps to obtain a more pure Se-enriched Ganoderma mycelia polysaccharides. The selenium polysaccharide was tested chemical properties. Selenium polysaccharide purification processing carried out two experiments on immune function, it is the first to observe Se-enriched Ganoderma lucidum polysaccharides on proliferation of nasopharyngeal carcinoma cells in vitro; the second is derived from human peripheral blood lymphocyte proliferation assay, to observe the effects of the selenium polysaccharides on immune function. The results show that the role of Se-enriched Ganoderma lucidum polysaccharides on immune function of these two experiments are stronger than Ganoderma lucidum polysaccharides not the accumulation of selenium. Finally fermentation broth its own characteristics, developed a fermentation broth with Se-enriched raw materials of health jelly.