| Purpose:This experiment adopts the chronic restraint stress model (CRS) to simulate human Symptoms of depression to studythe CRS SD rats pathological process and from many ways including behavior Lipid metabolism and inflammation to elaborate the mechanism of acupuncture intervention on depression.Method:1.Experimental groups:48 male SD rats were divided into 4 groups:control group, model group, acupuncture group and fluoxetine group according to the random number table. The rats in each group were fed for one week before the experiment.2. Modeling methods:In this study, we established the animal model of chronic psychological stress by using the internationally recognized chronic restraint stress rat model. The specific method:using barbed wire, Both ends of which are fixed by a clamp. The aim was to keep the breathing unobstructed, and not to oppress the rats. The rats were only subjected to psychological stress, which basically controlled the effect of physiological stress. The results mainly reflected the effect of psychological and emotional factors on the rats. Restraint stress time for the daily 9:00-15:00, during the period of restraint, intake and water deprivation. After the end of the shackles, the rats were put into mouse cage, free food intake.28 days in a row.3. Intervention methods:(1) Control group:conventional breeding for 28 days, only in the 9:00-15:00 daily intake and water deprivation, the rest of the time free feeding water and food.(2) Model group:chronic restraint combined with isolated for 28 days, restraint stress time for the daily 9:00-15:00, during the period of restraint, intake and water deprivation, the rest of the time free feeding water and food.(3) Acupuncture group:chronic restraint combined with isolated for 28 days, restraint stress time for the daily 9:00-15:00, during the period of restraint stress, intake and water deprivation, the rest of the time free feeding water and food.At the same time, selecting the "Baihui", "Yintang" and "Sanyinjiao" points to acupuncture intervention,1 hour before restraint stress retaining needle for 20 minutes, once a day, continuous 28 days.(4) Fluoxetine group:chronic restraint combined with isolated for 28 days, restraint stress time for the daily 9:00-15:00, during the period of restraint stress, intake and water deprivation, the rest of the time free feeding water and food. At the same time,using fluoxetine administered intervention 30 minutes before restraint stress, once a day, continuous 28 days.4. Detection index and methods:Using open field test, body weight and sucrose preference test to evaluate model rats behavior and using enzyme-linked immuosorbent assay to detect the expression of Apo-A1、Apo-D and RBP in each group at the end of the experiment (day28).Result:1. Effects on rat behavior:Before the experiment, there was no significant difference in each groups.(P>0.05) After 28days stress procedure, the model group showed a significant decrease of the number of crossing and rearing, sucrose consumption, sucrose preference index and the body weight compared to the control group (P<0.05); compared with the model group, acupuncture group showed a significant increase of the number of crossing and rearing, body weight, sucrose consumption and sucrose preference index (all P<0.05); compared with the model group, fluoxetine group showed a significant increase of the body weight, sucrose consumption and sucrose preference index(all P<0.05); compared with the fluoxetine group, acupuncture group showed a significant increase of the number of crossing and rearing(all P<0.05), the improvement effect of body weight was significantly better than that of fluoxetine.2.Effects on the expression level of Apo-A1、Apo-D and RBP in the cerebrospinal fluid:After 28days stress procedure, the model group showed a significant decrease of the expression level ofApo-A1、Apo-D and RBP in the cerebrospinal fluid compared to the control group (all P<0.05); compared with the model group, acupuncture group and fluoxetine group showed a significant increase of the expression level of Apo-A1、Apo-D and RBP in the cerebrospinal fluid (all P<0.05). There was no significant difference between the acupuncture group and fluoxetine group (all P>0.05)Conclusion:1. The combination of solitary and chronic restraint stress successfully simulated the behavior of human depression, acupuncture and fluoxetine intervention can significantly improve the behavior indicators of model rats, but Acupuncture was superior to fluoxetine in the improvement of exploratory behavior and physical activity status.2. The combination of solitary and chronic restraint stress stimulation leads to the expression level of Apo-A1、Apo-D and RBP in the cerebrospinal fluid were significantly decreased. Acupuncture intervention can effectively increase the expression level of Apo-A1、Apo-D and RBP in the cerebrospinal fluid of rats. |
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